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Antibodies
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anti-B-Cell CLL/lymphoma 2 (BCL2) (BH3 Domain Specific) antibody
B-Cell CLL/lymphoma 2 (BCL2) (BH3 Domain Specific) antibody
| Antigen |
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| Synonyms |
Bcl-2, bcl-2, BCL2 |
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Epitope
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| Clonality |
Polyclonal |
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Host
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Reactivity
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Human (145), Mouse (Murine) (57), Rat (Rattus) (55), Cow (Bovine) (37), Dog (Canine) (37), Pig (Porcine) (34), Rabbit (21), Chicken (18), Horse (Equine) (18), Cat (Feline) (2), Sheep (Ovine) (2)
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Application
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 Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), ELISA
Western Blotting (WB) (96), Immunofluorescence (IF) (66), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (47), Flow Cytometry (FACS) (43), Immunoprecipitation (IP) (35), Immunohistochemistry (IHC) (34), Immunohistochemistry (Frozen Sections) (IHC (fro)) (27), ELISA (23), Enzyme Immunoassay (EIA) (16), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Immunocytochemistry (ICC) (3), Dot Blot (Dot) (2), Immunoelectron Microscopy (IEM) (2)
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| Catalog no. |
ABIN388095 |
| Quantity |
0.1 mg (0.25 mg/ml) |
| Price |
383.50 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Purified Rabbit Polyclonal Antibody (Pab)
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Alternative name
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Bcl-2 (BH3 Domain Specific)
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Gene ID
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596
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Swiss-Prot
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P10415
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Immunogen
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This Bcl-2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 85~115 amino acids from the BH3 domain of human Bcl2.
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Isotype
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Ig (Matching secondary antibodies)
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Description
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Other names: Apoptosis regulator Bcl-2, B-cell lymphoma protein 2
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Specificity
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the BH3 domain of human Bcl2.
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Molecular Weight
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26266 DA
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Application Notes
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ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:10~50 Immunoprecipitation~~1:100~500 Flow cytometry~~1:100~500
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Concentration
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0.25 mg/ml
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Purification
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Purified
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Buffer
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage
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Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles
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Research Area
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Apoptosis/Necrosis, Cell Structure, Cancer
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Restrictions
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For Research Use only
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Publications
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Zheng, Zhou, Ye et al.: "The conformation change of Bcl-2 is involved in arsenic trioxide-induced apoptosis and inhibition of proliferation in SGC7901 human gastric cancer cells." in: World journal of surgical oncology, Vol. 8, Issue 1, pp. 31, 2010 (PubMed).
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Alternatives for antigen "B-Cell CLL/lymphoma 2 (BCL2)", type "Antibodies"
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Hosts
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Rabbit (123), Mouse (27), Goat (4), Sheep (3)
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Reactivities
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Human (145), Mouse (Murine) (57), Rat (Rattus) (55), Cow (Bovine) (37), Dog (Canine) (37), Pig (Porcine) (34), Rabbit (21), Chicken (18), Horse (Equine) (18), Cat (Feline) (2), Sheep (Ovine) (2)
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Applications
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Western Blotting (WB) (96), Immunofluorescence (IF) (66), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (47), Flow Cytometry (FACS) (43), Immunoprecipitation (IP) (35), Immunohistochemistry (IHC) (34), Immunohistochemistry (Frozen Sections) (IHC (fro)) (27), ELISA (23), Enzyme Immunoassay (EIA) (16), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Immunocytochemistry (ICC) (3), Dot Blot (Dot) (2), Immunoelectron Microscopy (IEM) (2)
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Conjugates
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PE (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2)
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Epitopes
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pSer70 (5), pThr56 (5)
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