BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11) (BH3 Domain) antibody

Antigen: BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11)

Synonyms: BAM, BIM, BOD, BimL, BimEL, BIM-beta6, BIM-beta7, BIM-alpha6, Bim, Bod, 1500006F24Rik, bim

Binding Site: BH3 Domain

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388101

Additional Information

Alternative name: Bim

Gene ID: 10018

UniProt: O43521

Immunogen: This Bim BH3 Domain antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide encoding the BH3 domain of human Bim.

Isotype: Ig

Description: Other names: Bcl-2-like protein 11, Bcl2 interacting mediator of cell death

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Bim BH3 Domain antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide encoding the BH3 domain of human Bim.

Molecular Weight: 22171 DA

Comments:

Background: Bim belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. Bim contains a Bcl-2 homology domain 3 (BH3). It has been shown to interact with other members of the BCL-2 protein family, including BCL2, BCL2L1/BCL-X(L), and MCL1, and to act as an apoptotic activator. The expression of this gene can be induced by nerve growth factor (NGF), as well as by the forkhead transcription factor FKHR-L1, which suggests a role of this gene in neuronal and lymphocyte apoptosis. Transgenic studies of the mouse counterpart suggested that this gene functions as an essential initiator of apoptosis in thymocyte-negative selection.

Application Details

Application Notes: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Apoptosis/Necrosis, Cancer, Cell Cycle, Transcription Factors, Signaling, Neurology, Cell Structure

Restrictions: For Research Use only

Images

The anti-Bim BH3 domain Pab (ABIN388101) is used in Western blot to detect Bim BH3 in HL-60 cell lysate.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Chen, Wang, Zhou et al.: "HIV-1 Tat targets microtubules to induce apoptosis, a process promoted by the pro-apoptotic Bcl-2 relative Bim." in: The EMBO journal, Vol. 21, Issue 24, pp. 6801-10, 2002 (PubMed).

Reginato, Mills, Paulus et al.: "Integrins and EGFR coordinately regulate the pro-apoptotic protein Bim to prevent anoikis." in: Nature cell biology, Vol. 5, Issue 8, pp. 733-40, 2003 (PubMed).

Sunters, Fernández de Mattos, Stahl et al.: "FoxO3a transcriptional regulation of Bim controls apoptosis in paclitaxel-treated breast cancer cell lines." in: The Journal of biological chemistry, Vol. 278, Issue 50, pp. 49795-805, 2003 (PubMed).

Luciano, Jacquel, Colosetti et al.: "Phosphorylation of Bim-EL by Erk1/2 on serine 69 promotes its degradation via the proteasome pathway and regulates its proapoptotic function." in: Oncogene, Vol. 22, Issue 43, pp. 6785-93, 2003 (PubMed).

Chen, Zhou: "Caspase cleavage of BimEL triggers a positive feedback amplification of apoptotic signaling." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 5, pp. 1235-40, 2004 (PubMed).