|+1 404 474 4654|
|+1 888 205 9894 (TF)|
BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11) (BH3 Domain) antibody
|Synonyms||BAM, BIM, BOD, BimL, BimEL, BIM-beta6, BIM-beta7, BIM-alpha6, Bim, Bod, 1500006F24Rik, bim|
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), ELISA
|5 references available|
|Quantity||400 µL (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This Bim BH3 Domain antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide encoding the BH3 domain of human Bim.|
|Description||Other names: Bcl-2-like protein 11, Bcl2 interacting mediator of cell death|
|Characteristics||Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This Bim BH3 Domain antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide encoding the BH3 domain of human Bim.|
|Molecular Weight||22171 DA|
Background: Bim belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. Bim contains a Bcl-2 homology domain 3 (BH3). It has been shown to interact with other members of the BCL-2 protein family, including BCL2, BCL2L1/BCL-X(L), and MCL1, and to act as an apoptotic activator. The expression of this gene can be induced by nerve growth factor (NGF), as well as by the forkhead transcription factor FKHR-L1, which suggests a role of this gene in neuronal and lymphocyte apoptosis. Transgenic studies of the mouse counterpart suggested that this gene functions as an essential initiator of apoptosis in thymocyte-negative selection.
|Application Notes||ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Apoptosis/Necrosis, Cancer, Cell Cycle, Transcription Factors, Signaling, Neurology, Cell Structure|
|Restrictions||For Research Use only|
Chen, Wang, Zhou et al.: "HIV-1 Tat targets microtubules to induce apoptosis, a process promoted by the pro-apoptotic Bcl-2 relative Bim." in: The EMBO journal, Vol. 21, Issue 24, pp. 6801-10, 2002 (PubMed).
Reginato, Mills, Paulus et al.: "Integrins and EGFR coordinately regulate the pro-apoptotic protein Bim to prevent anoikis." in: Nature cell biology, Vol. 5, Issue 8, pp. 733-40, 2003 (PubMed).
Sunters, Fernández de Mattos, Stahl et al.: "FoxO3a transcriptional regulation of Bim controls apoptosis in paclitaxel-treated breast cancer cell lines." in: The Journal of biological chemistry, Vol. 278, Issue 50, pp. 49795-805, 2003 (PubMed).
Luciano, Jacquel, Colosetti et al.: "Phosphorylation of Bim-EL by Erk1/2 on serine 69 promotes its degradation via the proteasome pathway and regulates its proapoptotic function." in: Oncogene, Vol. 22, Issue 43, pp. 6785-93, 2003 (PubMed).
Chen, Zhou: "Caspase cleavage of BimEL triggers a positive feedback amplification of apoptotic signaling." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 5, pp. 1235-40, 2004 (PubMed).