|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Apolipoprotein B MRNA Editing Enzyme, Catalytic Polypeptide-Like 3G (APOBEC3G) (Center,Glu133) antibody
|Synonyms||ARP9, CEM15, MDS019, FLJ12740, bK150C2.7, dJ494G10.1, APOBEC3G|
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), ELISA
|5 references available|
|Quantity||400 µL (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Alternative name||APOBEC3G (CEM15)|
|Immunogen||This CEM15 antibody is generated from mice immunized with KLH conjugated synthetic peptides corresponding to middle region of human CEM15.|
|Description||Other names: DNA dC->dU-editing enzyme APOBEC-3G, APOBEC-related cytidine deaminase, ARCD, APOBEC-related protein, ARP-9, CEM15, CEM-15, APOBEC3G, MDS019|
|Characteristics||Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This CEM15 antibody is generated from mice immunized with KLH conjugated synthetic peptides corresponding to middle region of human CEM15.|
|Molecular Weight||46408 DA|
Background: CEM15 is a member of the cytidine deaminase family. It is the product of one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. CEM15 has been found to be a specific inhibitor of human immunodeficiency virus-1 (HIV-1) infectivity.
|Application Notes||The suggested dilution will be: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Cell Cycle, Signaling, Chromatin, Cell Structure|
|Restrictions||For Research Use only|
Wedekind, Dance, Sowden et al.: "Messenger RNA editing in mammals: new members of the APOBEC family seeking roles in the family business." in: Trends in genetics : TIG, Vol. 19, Issue 4, pp. 207-16, 2003 (PubMed).
Zhang, Yang, Pomerantz et al.: "The cytidine deaminase CEM15 induces hypermutation in newly synthesized HIV-1 DNA." in: Nature, Vol. 424, Issue 6944, pp. 94-8, 2003 (PubMed).
Mangeat, Turelli, Caron et al.: "Broad antiretroviral defence by human APOBEC3G through lethal editing of nascent reverse transcripts." in: Nature, Vol. 424, Issue 6944, pp. 99-103, 2003 (PubMed).
Stopak, de Noronha, Yonemoto et al.: "HIV-1 Vif blocks the antiviral activity of APOBEC3G by impairing both its translation and intracellular stability." in: Molecular cell, Vol. 12, Issue 3, pp. 591-601, 2003 (PubMed).
Kao, Khan, Miyagi et al.: "The human immunodeficiency virus type 1 Vif protein reduces intracellular expression and inhibits packaging of APOBEC3G (CEM15), a cellular inhibitor of virus infectivity." in: Journal of virology, Vol. 77, Issue 21, pp. 11398-407, 2003 (PubMed).
|Hosts||Rabbit (30), Mouse (6), Goat (5)|
|Reactivities||Human (38), Mouse (Murine) (3), Rat (Rattus) (3), Cow (Bovine) (2), Dog (Canine) (2), Cat (Feline) (1), Chicken (1), Rabbit (1)|
|Applications||Western Blotting (WB) (38), ELISA (28), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (16), Immunohistochemistry (IHC) (11), Immunofluorescence (IF) (2), Immunoprecipitation (IP) (2)|
|Epitopes||N-Term (9), C-Term (4), AA 367-384 (2), Internal Region (2), Center,Asp143 (1)|