APOBEC3G antibody (AA 51-100)

Catalog No. ABIN6736355

Product Details anti-APOBEC3G Antibody

Target: APOBEC3G (Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3G (APOBEC3G))

Binding Specificity: AA 51-100

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APOBEC3G antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human APOBEC3G

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa51-100 of human ASGR2 (NP_068594). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%), Orangutan, Baboon, Monkey (92%), Marmoset (85%), Gibbon (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for APOBEC3G

Target: APOBEC3G (Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3G (APOBEC3G))

Alternative Name: CEM15 / APOBEC3G (APOBEC3G Products)

Synonyms: A3G antibody, ARCD antibody, ARP-9 antibody, ARP9 antibody, CEM-15 antibody, CEM15 antibody, bK150C2.7 antibody, dJ494G10.1 antibody, apolipoprotein B mRNA editing enzyme catalytic subunit 3G antibody, APOBEC3G antibody

Background: Name/Gene ID: APOBEC3G

Synonyms: APOBEC3G, ARCD, ARP-9, APOBEC-related protein 9, APOBEC-related protein, CEM-15, CEM15, Deoxycytidine deaminase, Phorbolin-like protein MDS019, ARP9, BK150C2.7, DJ494G10.1, MDS019

Gene ID: 60489

NCBI Accession: NP_068594

UniProt: Q9HC16