LC3 (APG8A) (AA 91-117), (Asp106) antibody

Details for Product No. ABIN388467
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Antigen
Epitope
AA 91-117, Asp106
(1), (1)
Reactivity
Human
(2), (1), (1)
Host
Rabbit
(2)
Clonality (Clone)
Polyclonal ()
Application
Immunofluorescence (IF), Western Blotting (WB)
(2), (2), (1)
Pubmed 2 references available
Catalog no. ABIN388467
Quantity 400 µL
Price
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Immunogen This LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 91-117 AA from human LC3.
Clone RB13262
Isotype Ig
Specificity This LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 92~121 amino acids from the center region of human LC3 (APG8a).
Predicted Reactivity Cow (Bovine),Mouse (Murine),Rat (Rattus)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Background Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3a is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.
Synonyms: MAP1LC3A, microtubule-associated protein 1 light chain 3 alpha, RP11-346K17.1, LC3, LC3A, MAP1ALC3, MAP1BLC3, MAP1 light chain 3-like protein 1, MAP1A/1B light chain 3 A, MAP1A/MAP1B LC3 A, MAP1A/MAP1B light chain 3 A, OTTHUMP00000030698, autophagy-relate
Molecular Weight 14272 DA
Gene ID 84557
UniProt Q9H492
Research Area Cell Structure, Autophagy
Application Notes IF = 1:100, WB = 1:1000
Restrictions For Research Use only
Format Liquid
Concentration 0.33 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-LC3 (APG8A) (AA 91-117), (Asp106) antibody Western blot analysis of LC3 (APG8a) (arrow) using rabbit polyclonal Autophagy LC3 Antibody (APG8a) (D106) (ABIN388467). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the LC3 (APG8a) gene (Lane 2) (Origene Technologies).
anti-LC3 (APG8A) (AA 91-117), (Asp106) antibody (2) SY5Y cells were pretreated with 5nM bafilomycin for 24hr and fixed in methanol . Treatment with ABIN388467 antibody at dilution 1:100. Data courtesy of Jianhui Zhu, MD, PhD & Charleen T. Chu, MD, PhD, University of Pittsburgh School of Medicine.
Background publications He, Dang, Dai et al.: "Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B." in: The Journal of biological chemistry, Vol. 278, Issue 31, pp. 29278-87, 2003 (PubMed).

Tanida, Sou, Ezaki et al.: "HsAtg4B/HsApg4B/autophagin-1 cleaves the carboxyl termini of three human Atg8 homologues and delipidates microtubule-associated protein light chain 3- and GABAA receptor-associated protein-phospholipid conjugates." in: The Journal of biological chemistry, Vol. 279, Issue 35, pp. 36268-76, 2004 (PubMed).

Hosts (2)
Reactivities (2), (1), (1)
Applications (2), (2), (1)
Epitopes (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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