LC3 (APG8B) (AA 77-106), (C-Term) antibody

Details for Product No. ABIN388474
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Antigen
Epitope
AA 77-106, C-Term
(2), (1), (1)
Reactivity
Human
(2), (1), (1)
Host
Rabbit
(2)
Clonality (Clone)
Polyclonal ()
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(2), (1), (1)
Pubmed 17 references available
Quantity 400 μL
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Immunogen This LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77-106 AA from the C-terminal region of human LC3.
Clone RB7485
Isotype Ig
Specificity This LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 88~118 amino acids from the C-term of human LC3 (APG8b).
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Background Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3b is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.
Synonyms: MAP1LC3B, LC3B, MAP1A/1BLC3, MAP1LC3B
Molecular Weight 14557 DA
Gene ID 81631
UniProt Q9GZQ8
Research Area Cell Signaling, Cell Structure, Autophagy
Application Notes WB = 1:1000, IHC (p) = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-LC3 (APG8B) (AA 77-106), (C-Term) antibody Western blot analysis of anti-LC3 (APG8b) Pab (ABIN388474) in 293 cell line lysates t...
anti-LC3 (APG8B) (AA 77-106), (C-Term) antibody (2) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
anti-LC3 (APG8B) (AA 77-106), (C-Term) antibody (3) Formalin-fixed and paraffin-embedded human testis tissue reacted with Autophagy LC3 a...
Product cited in: Ryu, Kim, Yeo et al.: "p65/RelA-Ser529 NF-κB subunit phosphorylation induces autophagic astroglial death (Clasmatodendrosis) following status epilepticus." in: Cellular and molecular neurobiology, Vol. 31, Issue 7, pp. 1071-8, 2011 (PubMed).

Bartlett, Isakson, Lewerenz et al.: "p62, Ref(2)P and ubiquitinated proteins are conserved markers of neuronal aging, aggregate formation and progressive autophagic defects." in: Autophagy, Vol. 7, Issue 6, pp. 572-83, 2011 (PubMed).

Li, Wang, Fei et al.: "Parkinson's disease involves autophagy and abnormal distribution of cathepsin L." in: Neuroscience letters, Vol. 489, Issue 1, pp. 62-7, 2011 (PubMed).

Zhuang, Li, Long et al.: "Knockdown of the DNA-dependent protein kinase catalytic subunit radiosensitizes glioma-initiating cells by inducing autophagy." in: Brain research, Vol. 1371, pp. 7-15, 2011 (PubMed).

Arnold, Cassady, VanLaar et al.: "Integrating multiple aspects of mitochondrial dynamics in neurons: age-related differences and dynamic changes in a chronic rotenone model." in: Neurobiology of disease, Vol. 41, Issue 1, pp. 189-200, 2010 (PubMed).

Cherra, Kulich, Uechi et al.: "Regulation of the autophagy protein LC3 by phosphorylation." in: The Journal of cell biology, Vol. 190, Issue 4, pp. 533-9, 2010 (PubMed).

Yang, Liu, Goga et al.: "Therapeutic potential of a synthetic lethal interaction between the MYC proto-oncogene and inhibition of aurora-B kinase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 31, pp. 13836-41, 2010 (PubMed).

Wang, Gorvel, Chu et al.: "Helicobacter pylori impairs murine dendritic cell responses to infection." in: PLoS ONE, Vol. 5, Issue 5, pp. e10844, 2010 (PubMed).

Eng, Panas, Karlsson Hedestam et al.: "A novel quantitative flow cytometry-based assay for autophagy." in: Autophagy, Vol. 6, Issue 5, pp. 634-41, 2012 (PubMed).

Ren, He, Nong et al.: "Acquired cisplatin resistance in human lung adenocarcinoma cells is associated with enhanced autophagy." in: Cancer biotherapy & radiopharmaceuticals, Vol. 25, Issue 1, pp. 75-80, 2010 (PubMed).

Caramés, Taniguchi, Otsuki et al.: "Autophagy is a protective mechanism in normal cartilage, and its aging-related loss is linked with cell death and osteoarthritis." in: Arthritis and rheumatism, Vol. 62, Issue 3, pp. 791-801, 2010 (PubMed).

Calakos, Patel, Gottron et al.: "Functional evidence implicating a novel TOR1A mutation in idiopathic, late-onset focal dystonia." in: Journal of medical genetics, Vol. 47, Issue 9, pp. 646-50, 2010 (PubMed).

Background publications Baehrecke: "Autophagy: dual roles in life and death?" in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 505-10, 2005 (PubMed).

Lum, DeBerardinis, Thompson: "Autophagy in metazoans: cell survival in the land of plenty." in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 439-48, 2005 (PubMed).

Shintani, Klionsky: "Autophagy in health and disease: a double-edged sword." in: Science (New York, N.Y.), Vol. 306, Issue 5698, pp. 990-5, 2004 (PubMed).

Tanida, Ueno, Kominami: "LC3 conjugation system in mammalian autophagy." in: The international journal of biochemistry & cell biology, Vol. 36, Issue 12, pp. 2503-18, 2004 (PubMed).

He, Dang, Dai et al.: "Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B." in: The Journal of biological chemistry, Vol. 278, Issue 31, pp. 29278-87, 2003 (PubMed).

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