ATG4 Autophagy Related 4 Homolog A (S. Cerevisiae) (ATG4A) (AA 363-392) antibody

Details for Product No. ABIN388493
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Antigen
Epitope
AA 363-392
(12), (5), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(56), (28), (14), (13), (1), (1)
Host
Rabbit
(54), (2), (1)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(30), (20), (18), (12), (10), (8), (2), (2), (1)
Pubmed 5 references available
Quantity 400 μL
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Catalog No. ABIN388493
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Immunogen This ATG4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 363-392 AA from human ATG4A.
Clone RB7555
Isotype Ig
Specificity This ATG4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 369~398 amino acids from the C-term of human APG4A.
Predicted Reactivity Cow (Bovine)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name ATG4A
Background Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG4A is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP.
Synonyms: RP5-889N15.2, APG4A, AUTL2, APG4 autophagy 4 homolog A, AUT-like 2 cysteine endopeptidase, AUT-like 2, cysteine endopeptidase, autophagin 2, autophagin-2, autophagy-related cysteine endopeptidase 2, autophagy-related protein 4 homolog A, cysteine protease
Molecular Weight 45378 DA
Gene ID 115201
UniProt Q8WYN0
Research Area Cell Signaling, Cell Structure, Autophagy
Application Notes WB = 1:1000, IHC = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-ATG4 Autophagy Related 4 Homolog A (S. Cerevisiae) (ATG4A) (AA 363-392) antibody Western blot analysis of anti-APG4A Pab (ABIN388493) in Jurkat cell line lysates (35 µg/lane). APG4A (arrow) was detected using the purified polyclonal antibody.
anti-ATG4 Autophagy Related 4 Homolog A (S. Cerevisiae) (ATG4A) (AA 363-392) antibody (2) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
anti-ATG4 Autophagy Related 4 Homolog A (S. Cerevisiae) (ATG4A) (AA 363-392) antibody (3) Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with Autophagy APG4A Antibody (C-term) (ABIN388493), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Background publications Shintani, Klionsky: "Autophagy in health and disease: a double-edged sword." in: Science (New York, N.Y.), Vol. 306, Issue 5698, pp. 990-5, 2004 (PubMed).

Levine: "Eating oneself and uninvited guests: autophagy-related pathways in cellular defense." in: Cell, Vol. 120, Issue 2, pp. 159-62, 2005 (PubMed).

Lum, DeBerardinis, Thompson: "Autophagy in metazoans: cell survival in the land of plenty." in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 439-48, 2005 (PubMed).

Baehrecke: "Autophagy: dual roles in life and death?" in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 505-10, 2005 (PubMed).

Greenberg: "Degrade or die: a dual function for autophagy in the plant immune response." in: Developmental cell, Vol. 8, Issue 6, pp. 799-801, 2005 (PubMed).

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