ATG4B antibody (ATG4 Autophagy Related 4 Homolog B (S. Cerevisiae)) (AA 258-284)

Details for Product anti-ATG4B Antibody No. ABIN388497, Supplier: Log in to see
Antigen
  • apg4b
  • APG4B
  • AUTL1
  • 2510009N07Rik
  • AW048066
  • Apg4b
  • Atg4bl
  • Autl1
  • Aut2b2
  • autophagy related 4B, cysteine peptidase
  • autophagy-related 4b
  • cysteine protease ATG4b
  • cysteine protease ATG4B
  • ATG4 autophagy related 4 homolog B (S. cerevisiae)
  • ATG4B
  • Atg4b
  • AT3G59950
  • Tsp_08582
  • Tsp_11599
  • atg4b
Epitope
AA 258-284
48
22
18
18
16
15
12
10
9
8
7
5
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
175
100
46
10
10
8
8
7
7
6
5
4
4
3
2
2
1
1
Host
Rabbit
170
7
1
Clonality (Clone)
Polyclonal ()
Conjugate
This ATG4B antibody is un-conjugated
7
7
7
6
6
6
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
154
50
43
42
38
25
13
2
2
1
Options
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Immunogen This ATG4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 258-284 AA from human ATG4B.
Clone RB11843
Isotype Ig
Specificity This ATG4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 264~293 amino acids from the central region of human APG4B.
Predicted Reactivity Cow (Bovine),Chicken,Mouse (Murine),Zebrafish
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name ATG4B (ATG4B Antibody Abstract)
Background Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG4, a cysteine protease required for autophagy, cleaves the C-terminal part of MAP1LC3 to form the activated molecule LC3-I. LC3-I is subsequently conjugated with phosphatidylethanolamine at the C-terminal glycine to form LC3-II, a marker for autophagy via its capacity to bind to autophagosomes.
Synonyms: APG4B, AUTL1, MGC1353, APG4 autophagy 4 homolog B, AUT-like 1 cysteine endopeptidase, autophagin-1, autophagy-related cysteine endopeptidase 1, autophagy-related protein 4 homolog B, cysteine protease ATG4B, hAPG4B
Molecular Weight 44309 DA
Gene ID 23192
UniProt Q9Y4P1
Research Area Cell Structure, Autophagy
Application Notes WB = 1:1000, IHC (p) = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-ATG4B antibody (ATG4 Autophagy Related 4 Homolog B (S. Cerevisiae)) (AA 258-284) (ABIN388497) Western blot analysis of APG4B (arrow) using purified polyclonal antibody (ABIN388497...
Immunohistochemistry (IHC) image for anti-ATG4B antibody (ATG4 Autophagy Related 4 Homolog B (S. Cerevisiae)) (AA 258-284) (ABIN388497) Formalin-fixed and paraffin-embedded human skeletal muscle reacted with Autophagy APG...
Background publications Tanida, Sou, Ezaki, Minematsu-Ikeguchi, Ueno, Kominami: "HsAtg4B/HsApg4B/autophagin-1 cleaves the carboxyl termini of three human Atg8 homologues and delipidates microtubule-associated protein light chain 3- and GABAA receptor-associated protein-phospholipid conjugates." in: The Journal of biological chemistry, Vol. 279, Issue 35, pp. 36268-76, 2004 (PubMed).

Mariño, Uría, Puente, Quesada, Bordallo, López-Otín: "Human autophagins, a family of cysteine proteinases potentially implicated in cell degradation by autophagy." in: The Journal of biological chemistry, Vol. 278, Issue 6, pp. 3671-8, 2003 (PubMed).