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ATG7 Autophagy Related 7 Homolog (S. Cerevisiae) (ATG7) (AA 284-313), (Center) antibody

Details for Product No. ABIN388521, Supplier: Log in to see
Antigen
  • AO090001000744
  • atg7
  • APG7-LIKE
  • APG7L
  • GSA7
  • 1810013K23Rik
  • Agp7
  • Apg7l
  • Atg7l
  • ATAPG7
  • ATATG7
  • ATG7
  • AUTOPHAGY 7
  • AUTOPHAGY-RELATED 7
  • K15I22.10
  • K15I22_10
Epitope
AA 284-313, Center
29
16
15
14
13
8
7
5
4
3
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
143
41
25
Host
Rabbit
137
10
3
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
4
4
4
4
4
4
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
114
62
55
31
20
16
8
6
6
3
3
2
1
1
Supplier
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Immunogen This ATG7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 284-313 AA from the Central region of human ATG7.
Clone RB7469
Isotype Ig
Specificity This ATG7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 291~321 amino acids from the Center of human APG7L.
Predicted Reactivity Chicken,Rat (Rattus)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name ATG7 (ATG7 Antibody Abstract)
Background Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG7 functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. APG3L is an E2-like conjugating enzyme facilitating covalent binding of APG8 (MAP1LC3) to phosphatidylethanolamine (PE). APG7 (an E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with APG3. Formation of the PE conjugate is essential for autophagy.
Synonyms: APG5, APG5-LIKE, APG5L, ASP, hAPG5, APG5 (autophagy 5, S. cerevisiae)-like, APG5 autophagy 5-like, ATG5 autophagy related 5-like, OTTHUMP00000017824, apoptosis specific protein, apoptosis-specific protein, autophagy protein 5
Molecular Weight 77960 DA
Gene ID 10533
UniProt O95352
Research Area Cell Signaling, Cell Structure, Autophagy
Application Notes WB = 1:1000, IHC (p) = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-ATG7 Autophagy Related 7 Homolog (S. Cerevisiae) (ATG7) (AA 284-313), (Center) antibody (ABIN388521) Western blot analysis of anti-hAPG7L-P299 Pab (ABIN388521) in 293 cell line lysates t...
Western Blotting (WB) image for anti-ATG7 Autophagy Related 7 Homolog (S. Cerevisiae) (ATG7) (AA 284-313), (Center) antibody (ABIN388521) The anti-APG7L Pab (ABIN388522) is used in Western blot to detect APG7L in mouse live...
Immunohistochemistry (IHC) image for anti-ATG7 Autophagy Related 7 Homolog (S. Cerevisiae) (ATG7) (AA 284-313), (Center) antibody (ABIN388521) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
Product cited in: Ma, Molusky, Song et al.: "Autophagy deficiency by hepatic FIP200 deletion uncouples steatosis from liver injury in NAFLD." in: Molecular endocrinology (Baltimore, Md.), Vol. 27, Issue 10, pp. 1643-54, 2013 (PubMed).

Ma, Panda, Lin: "Temporal orchestration of circadian autophagy rhythm by C/EBP?." in: The EMBO journal, Vol. 30, Issue 22, pp. 4642-51, 2012 (PubMed).

Background publications Greenberg: "Degrade or die: a dual function for autophagy in the plant immune response." in: Developmental cell, Vol. 8, Issue 6, pp. 799-801, 2005 (PubMed).

Baehrecke: "Autophagy: dual roles in life and death?" in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 505-10, 2005 (PubMed).

Lum, DeBerardinis, Thompson: "Autophagy in metazoans: cell survival in the land of plenty." in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 439-48, 2005 (PubMed).

Levine: "Eating oneself and uninvited guests: autophagy-related pathways in cellular defense." in: Cell, Vol. 120, Issue 2, pp. 159-62, 2005 (PubMed).

Shintani, Klionsky: "Autophagy in health and disease: a double-edged sword." in: Science (New York, N.Y.), Vol. 306, Issue 5698, pp. 990-5, 2004 (PubMed).

Tanida, Tanida-Miyake, Nishitani et al.: "Murine Apg12p has a substrate preference for murine Apg7p over three Apg8p homologs." in: Biochemical and biophysical research communications, Vol. 292, Issue 1, pp. 256-62, 2002 (PubMed).

Tanida, Tanida-Miyake, Komatsu et al.: "Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the conjugation of hApg12p to hApg5p." in: The Journal of biological chemistry, Vol. 277, Issue 16, pp. 13739-44, 2002 (PubMed).