Beclin 1, Autophagy Related (BECN1) (AA 181-210) antibody

Details for Product No. ABIN388545
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Antigen
Synonyms ATG6, VPS30, beclin1, 4921513J16Rik, 5430417M23Rik, Atg6, zgc:73097, beclin-1, atg6, vps30, Beclin-1, becn1
Epitope
AA 181-210
(19), (17), (7), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(166), (81), (57), (32), (30), (21), (18), (15), (12), (6), (2), (1)
Host
Rabbit
(123), (54), (1), (1)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(6), (6), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(163), (55), (50), (47), (38), (23), (18), (14), (10), (5), (5), (3), (1)
Pubmed 6 references available
Catalog no. ABIN388545
Quantity 400 µL
Price
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Immunogen This Beclin 1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 181-210 AA from human Beclin 1.
Clone RB7461
Isotype Ig
Specificity This Beclin 1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 188~218 amino acids surrounding amino acid Q196 of human BECN1.
Predicted Reactivity Cow (Bovine),Chicken
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name Beclin 1
Background Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). Beclin 1 plays a role in two fundamentally important cell biological pathways: autophagy and apoptosis. Beclin 1 is thought to function as a VPS and autophagy protein as part of a complex with Class III PI3 kinase, Vps34.
Synonyms: BECN1, ATG6, VPS30, beclin1, ATG6 autophagy related 6 homolog, beclin 1 (coiled-coil, moesin-like BCL2 interacting protein), beclin 1 (coiled-coil, moesin-like BCL2-interacting protein), beclin-1, coiled-coil myosin-like BCL2-interacting protein
Molecular Weight 51896 DA
Gene ID 8678
UniProt Q14457
Research Area Apoptosis/Necrosis, Cell Signaling, Cell Structure, Autophagy
Application Notes WB = 1:1000, IHC = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Beclin 1, Autophagy Related (BECN1) (AA 181-210) antibody Western blot analysis of anti-hBECN1-Q196 Pab (ABIN388545) in 293 cell line lysates transiently transfected with the BECN1 gene (2 µg/lane). hBECN1-Q196(arrow) was detected using the purified polyclonal antibody (1:30 dilution).
anti-Beclin 1, Autophagy Related (BECN1) (AA 181-210) antibody (2) The anti-BECN1 Pab (ABIN388545) is used in Western blot to detect BECN1 in mouse liver tissue lysate. BECN1(arrow) was detected using the purified polyclonal antibody.
anti-Beclin 1, Autophagy Related (BECN1) (AA 181-210) antibody (3) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
Background publications Liang, Kleeman, Jiang et al.: "Protection against fatal Sindbis virus encephalitis by beclin, a novel Bcl-2-interacting protein." in: Journal of virology, Vol. 72, Issue 11, pp. 8586-96, 1998 (PubMed).

Shintani, Klionsky: "Autophagy in health and disease: a double-edged sword." in: Science (New York, N.Y.), Vol. 306, Issue 5698, pp. 990-5, 2004 (PubMed).

Levine: "Eating oneself and uninvited guests: autophagy-related pathways in cellular defense." in: Cell, Vol. 120, Issue 2, pp. 159-62, 2005 (PubMed).

Lum, DeBerardinis, Thompson: "Autophagy in metazoans: cell survival in the land of plenty." in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 439-48, 2005 (PubMed).

Baehrecke: "Autophagy: dual roles in life and death?" in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 505-10, 2005 (PubMed).

Greenberg: "Degrade or die: a dual function for autophagy in the plant immune response." in: Developmental cell, Vol. 8, Issue 6, pp. 799-801, 2005 (PubMed).

Hosts (123), (54), (1), (1)
Reactivities (166), (81), (57), (32), (30), (21), (18), (15), (12), (6), (2), (1)
Applications (163), (55), (50), (47), (38), (23), (18), (14), (10), (5), (5), (3), (1)
Conjugates (6), (6), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (19), (17), (7), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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