ATG14 Autophagy Related 14 Homolog (S. Cerevisiae) (ATG14) (N-Term), (AA 55-84) antibody

Details for Product No. ABIN388584
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Antigen
Epitope
N-Term, AA 55-84
(7), (7), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(19), (7), (7)
Host
Rabbit
(19)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Flow Cytometry (FACS)
(19), (13), (8), (5), (1), (1), (1)
Pubmed 1 reference available
Quantity 400 µL
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Catalog No. ABIN388584
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Immunogen This ATG14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 55-84 AA from the N-terminal region of human ATG14.
Clone RB19669
Isotype Ig
Specificity This ATG14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62~92 amino acids from the N-terminal region of human ATG14.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name Barkor
Background Synonyms: Uncharacterized protein KIAA0831, KIAA0831
Molecular Weight 55309 DA
Gene ID 1465
UniProt P21291
Research Area Autophagy
Application Notes WB = 1:1000, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-ATG14 Autophagy Related 14 Homolog (S. Cerevisiae) (ATG14) (N-Term), (AA 55-84) antibody Western blot analysis of Barkor antibody (N-term) (ABIN388584) in mouse heart tissue lysates (35 µg/lane). Barkor (arrow) was detected using the purified polyclonal antibody.
anti-ATG14 Autophagy Related 14 Homolog (S. Cerevisiae) (ATG14) (N-Term), (AA 55-84) antibody (2) Barkor Antibody (N-term) (ABIN388584) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Sun, Fan, Chen et al.: "Identification of Barkor as a mammalian autophagy-specific factor for Beclin 1 and class III phosphatidylinositol 3-kinase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 49, pp. 19211-6, 2008 (PubMed).

Validation Images
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