Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Predicted Reactivity
X
Purification
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen
This SMURF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-96 amino acids from the N-terminal region of human SMURF1.
Guo, Yamashita, Zhang, Zhou, Chen, Reynolds, Awad, Yanoso, Zhao, Schwarz, Zhang, Boyce, Xing: "Ubiquitin ligase Smurf1 mediates tumor necrosis factor-induced systemic bone loss by promoting proteasomal degradation of bone morphogenetic signaling proteins." in: The Journal of biological chemistry, Vol. 283, Issue 34, pp. 23084-92, (2008) (PubMed).
Schwamborn, Müller, Becker, Püschel: "Ubiquitination of the GTPase Rap1B by the ubiquitin ligase Smurf2 is required for the establishment of neuronal polarity." in: The EMBO journal, Vol. 26, Issue 5, pp. 1410-22, (2007) (PubMed).
Kaneki, Guo, Chen, Yao, Schwarz, Zhang, Boyce, Xing: "Tumor necrosis factor promotes Runx2 degradation through up-regulation of Smurf1 and Smurf2 in osteoblasts." in: The Journal of biological chemistry, Vol. 281, Issue 7, pp. 4326-33, (2006) (PubMed).
Target
SMURF1
(SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1))
4930431E10Rik antibody, mKIAA1625 antibody, RGD1309707 antibody, SMAD specific E3 ubiquitin protein ligase 1 antibody, E3 ubiquitin-protein ligase SMURF1 antibody, SMAD specific E3 ubiquitin protein ligase 1 L homeolog antibody, SMURF1 antibody, LOC100540915 antibody, smurf1 antibody, Smurf1 antibody, smurf1.L antibody
Background
Members of the transforming growth factor-beta (TGFB) family signal through type I and type II serine/threonine-kinase receptors, which in turn activate the SMAD signaling pathway. Bone morphogenetic protein (BMP) receptors target SMAD1, SMAD5, and SMAD8, whereas receptors for activin and TGFB (e.g., ACVR1 and TGFBR1, respectively) target SMAD2 and SMAD3. Phosphorylation of these receptor-regulated SMADs induces their association with the common-partner SMAD, SMAD4. Smurf1, a HECT domain E3 ubiquitin ligase, regulates cell polarity and protrusive activity and is required to maintain the transformed morphology and motility of a tumor cell. Atypical protein kinase C-zeta (PKC2), an effector of the Cdc42/Rac1-PAR6 polarity complex, recruits Smurf1 to cellular protrusions, where it controlled the local level of RhoA. Smurf1 thus links the polarity complex to degradation of RhoA in lamellipodia and filopodia to prevent RhoA signaling during dynamic membrane movements.