Ubiquitin Specific Peptidase 8 (USP8) (C-Term) antibody

Antigen: Ubiquitin Specific Peptidase 8 (USP8)

Synonyms: UBPY, HumORF8, FLJ34456, KIAA0055, MGC129718

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388891

Additional Information

Alternative name: USP8 (UBPY)

Gene ID: 9101

UniProt: UBP8_HUMAN

Immunogen: This USP8 (UBPY) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1065~1094 amino acids from the C-terminal region of human USP8.

Isotype: Ig

Description: Other names: Ubiquitin carboxyl-terminal hydrolase 8, Ubiquitin thiolesterase 8, Ubiquitin-specific processing protease 8, Deubiquitinating enzyme 8, hUBPy

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This USP8 (UBPY) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1065~1094 amino acids from the C-terminal region of human USP8.

Molecular Weight: 127523 DA

Comments:

Background: USP8 is a ubiquitin specific protease that plays an important regulatory role at the level of protein turnover by preventing degradation. USP8 is involved in cell proliferation, and probably regulates the stability of STAM2 and RASGRF1. USP8 may regulate T-cell anergy mediated by RNF128 via the formation of a complex containing RNF128 and STAM2. As revealed by structur/function studies, USP8 forms a ternary complex with RNF128 and OTUB1, and interacts with the SH3 domain of STAM2 and RASGRF1. Expression of USP8 is induced Upon growth stimulation in starved human fibroblasts, and expression decreases in response to growth arrest induced by cell-cell contact.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Ubiquitin-related antibodies, Protein Modifications, Cell Cycle, Cell Structure

Restrictions: For Research Use only

Images

The anti-USP8 Pab (ABIN388891) is used in Western blot to detect USP8 in transfected 293T cells. Data is kindly provided by Lily Yen from University of California Davis (Davis, CA).

USP8-C1072 (ABIN388891) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the USP8 antibody detected the USP8 protein (arrow).

Publications

Product: Naviglio, Mattecucci, Matoskova et al.: "UBPY: a growth-regulated human ubiquitin isopeptidase." in: The EMBO journal, Vol. 17, Issue 12, pp. 3241-50, 1998 (PubMed).

Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).