USP8 antibody (Ubiquitin Specific Peptidase 8) (C-Term)

Details for Product anti-USP8 Antibody No. ABIN357535, Supplier: Log in to see
Antigen
  • HumORF8
  • UBPY
  • AI574262
  • AW557536
  • Ubpy
  • mKIAA0055
  • ubiquitin carboxyl-terminal hydrolase 8
  • ubiquitin specific peptidase 8
  • LOC9310109
  • MGYG_00369
  • USP8
  • Usp8
Epitope
C-Term
26
21
13
13
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human
93
5
4
4
3
3
2
1
1
1
1
1
Host
Rabbit
84
6
2
1
Clonality
Polyclonal
Conjugate
This USP8 antibody is un-conjugated
6
6
6
6
6
6
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
87
48
45
10
9
7
4
3
2
2
1
Options
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Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human USP8.
Isotype Ig Fraction
Specificity This antibody is specific to USP8/UBPY (C-term). Predicted to cross react with Mouse (100 % Antigen Homology).
Purification Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Plasmids, Primers & others Plasmids, Primers & others USP8 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name USP8 / UBPY (USP8 Antibody Abstract)
Background USP8 is a ubiquitin specific protease that plays an important regulatory role at the level of protein turnover by preventing degradation. USP8 is involved in cell proliferation, and probably regulates the stability of STAM2 and RASGRF1. USP8 may regulate T-cell anergy mediated by RNF128 via the formation of a complex containing RNF128 and STAM2. As revealed by structur/function studies, USP8 forms a ternary complex with RNF128 and OTUB1, and interacts with the SH3 domain of STAM2 and RASGRF1. Expression of USP8 is induced Upon growth stimulation in starved human fibroblasts, and expression decreases in response to growth arrest induced by cell-cell contact.Synonyms: Deubiquitinating enzyme 8, KIAA0055, Ubiquitin carboxyl-terminal hydrolase 8, Ubiquitin thioesterase 8, Ubiquitin-specific-processing protease 8
Molecular Weight 127523 Da
Gene ID 9101, 5874
UniProt P40818
Application Notes ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Supplier Images
 image for anti-Ubiquitin Specific Peptidase 8 (USP8) (C-Term) antibody (ABIN357535) anti-Ubiquitin Specific Peptidase 8 (USP8) (C-Term) antibody
 image for anti-Ubiquitin Specific Peptidase 8 (USP8) (C-Term) antibody (ABIN357535) anti-Ubiquitin Specific Peptidase 8 (USP8) (C-Term) antibody (Image 2)
Background publications Strausberg, Feingold, Grouse, Derge, Klausner, Collins, Wagner, Shenmen, Schuler, Altschul, Zeeberg, Buetow, Schaefer, Bhat, Hopkins, Jordan, Moore, Max, Wang, Hsieh, Diatchenko, Marusina, Farmer et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. ..." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Naviglio, Mattecucci, Matoskova, Nagase, Nomura, Di Fiore, Draetta: "UBPY: a growth-regulated human ubiquitin isopeptidase." in: The EMBO journal, Vol. 17, Issue 12, pp. 3241-50, 1998 (PubMed).

Nomura, Nagase, Miyajima, Sazuka, Tanaka, Sato, Seki, Kawarabayasi, Ishikawa, Tabata: "Prediction of the coding sequences of unidentified human genes. II. The coding sequences of 40 new genes (KIAA0041-KIAA0080) deduced by analysis of cDNA clones from human cell line KG-1." in: DNA research : an international journal for rapid publication of reports on genes and genomes, Vol. 1, Issue 5, pp. 223-9, 1995 (PubMed).

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