Mindbomb Homolog 1 (Drosophila) (MIB1) (N-Term) antibody

Antigen: Mindbomb Homolog 1 (Drosophila) (MIB1)

Synonyms: MIB, ZZZ6, DIP-1, ZZANK2, FLJ90676, MGC129659, MGC129660, DKFZp686I0769, DKFZp761M1710, Mib, MGC18948, mKIAA1323, E430019M12Rik

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388956

Additional Information

Alternative name: Mib1/Mindbomb

Gene ID: 57534

UniProt: Q86YT6

Immunogen: This Mib1/Mindbomb antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15~45 amino acids from the N-terminal region of human MIB.

Isotype: Ig

Description: Other names: E3 ubiquitin-protein ligase MIB1, Mind bomb homolog 1, DAPK-interacting protein 1, DIP-1, Zinc finger ZZ type with ankyrin repeat domain protein 2, MIB1, DIP1, KIAA1323, ZZANK2

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Mib1/Mindbomb antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15~45 amino acids from the N-terminal region of human MIB.

Molecular Weight: 110135 DA

Comments:

Background: MIB is an E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. This protein positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. MIB probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western Blotting ~~ 1:100~500 Immunohistochemistry~~1:50~100 Immunofluorescence~~1:20~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Ubiquitin-related antibodies, Translation Factors, Cell Signaling, Protein Modifications, Cell Structure, Cell Cycle, Transcription Factors, Signaling, Neurology

Restrictions: For Research Use only

Images

The Mib Pab (ABIN388956) is used at 1:300 to detect FLAG-tagged Mib in 293T cell lysate. Data courtesy of Dr. Olga Ossipova, Mt. Sinai School of Medicine.

Immunofluorescent staining of Hela cells incubated with MIB Antibody (N-term) (ABIN388956) at a dilution of 1:20.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Jin, Blue, Dixon et al.: "A death-associated protein kinase (DAPK)-interacting protein, DIP-1, is an E3 ubiquitin ligase that promotes tumor necrosis factor-induced apoptosis and regulates the cellular levels of DAPK." in: The Journal of biological chemistry, Vol. 277, Issue 49, pp. 46980-6, 2002 (PubMed).

Itoh, Kim, Palardy et al.: "Mind bomb is a ubiquitin ligase that is essential for efficient activation of Notch signaling by Delta." in: Developmental cell, Vol. 4, Issue 1, pp. 67-82, 2003 (PubMed).