Heat Shock Factor Protein 1 (HSF1) (pSumoylation Site), (AA 278-309) antibody

Details for Product No. ABIN389069
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Antigen
Synonyms hsf1, Hsf, xhsf1, AA960185, HSTF1, HSF1, HSF, HSF1c, hsf1a, hsf1b, zHSF1a, zHSF1b, hstf1
Epitope
pSumoylation Site, AA 278-309
(89), (53), (23), (16), (14), (13), (11), (9), (8), (7), (7), (7), (6), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(284), (215), (87), (57), (41), (40), (40), (40), (24), (24), (2), (2), (1), (1), (1)
Host
Rabbit
(225), (112), (23), (1)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(11), (11), (11), (7), (7), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (2)
Application
Western Blotting (WB), Immunofluorescence (IF)
(294), (186), (117), (107), (106), (87), (49), (47), (40), (1), (1), (1)
Pubmed 1 reference available
Quantity 400 μL
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Catalog No. ABIN389069
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Immunogen This HSF1 Sumoylation Site antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 278-309 AA from human HSF1 Sumoylation Site.
Clone RB2562
Isotype Ig
Specificity This HSF1 Sumoylation Site antibody is generated from rabbits immunized with the KLH conjugated synthetic peptide PLVRVKEEPPSPPQSPR containing a predicted sumoylation site from the central region of human HSF1.
Predicted Reactivity Cow (Bovine)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name HSF1
Background Heat shock transcription factor 1 (HSF1) mediates the induction of heat shock protein gene expression in cells exposed to elevated temperature and other stress conditions. In response to stress, HSF1 acquires DNA-binding ability and localizes to nuclear stress granules. SUMO modification of HSF1 converts HSF1 to the DNA-binding form. HSF1 colocalizes with SUMO-1 in nuclear stress granules, which is prevented by mutation of the HSF1 lysine targeted for sumoylation.
Synonyms: Heat shock factor protein 1, HSF 1, Heat shock transcription factor 1, HSTF 1, HSF1, HSTF1
Molecular Weight 57260 Da
Gene ID 3297
UniProt Q00613
Research Area Apoptosis/Necrosis, Cell Signaling, Protein Modifications, Transcription Factors, Signaling, Cell Structure
Application Notes WB = 1:1000, IF = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Heat Shock Factor Protein 1 (HSF1) (pSumoylation Site), (AA 278-309) antibody Confocal immunofluorescent analysis of HSF1 Sumoylation Site Antibody (ABIN389069) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
anti-Heat Shock Factor Protein 1 (HSF1) (pSumoylation Site), (AA 278-309) antibody (2) Western blot analysis of HSF1 polyclonal antibody (ABIN389069) in 293 cell line lysates. Non-sumoylated HSF1 (arrow) was detected using the purified polyclonal antibody.
anti-Heat Shock Factor Protein 1 (HSF1) (pSumoylation Site), (AA 278-309) antibody (3) Western blot analysis of HSF1 (arrow) using rabbit polyclonal HSF1 Antibody (Sumo) (ABIN389069). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HSF1 gene.
Background publications Wang, Grammatikakis, Siganou et al.: "Regulation of molecular chaperone gene transcription involves the serine phosphorylation, 14-3-3 epsilon binding, and cytoplasmic sequestration of heat shock factor 1." in: Molecular and cellular biology, Vol. 23, Issue 17, pp. 6013-26, 2003 (PubMed).

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