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Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5) (AA 443-473), (Center) antibody

Details for Product No. ABIN389219
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Antigen
Synonyms lgr5-a, lgr5a, FEX, Gpr49, GPR49, GPR67, GRP49, HG38
Epitope
AA 443-473, Center
(7), (5), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(75), (29), (19), (17), (17), (17)
Host
Rabbit
(63), (14)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
(46), (34), (21), (17), (16), (14), (11), (11), (1)
Pubmed 1 reference available
Quantity 400 µL
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Catalog No. ABIN389219
291.50 $
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Immunogen This LGR5 (GPR49) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 443-473 AA from the Central region of human LGR5 (GPR49).
Clone RB14555
Isotype Ig
Specificity This LGR5 (GPR49) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 451~480 amino acids from the center region of human LGR5/GPR49.
Predicted Reactivity Cow (Bovine)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name LGR5 (GPR49)
Background LGR5/GPR49 is an orphan receptor. It may be an important receptor for signals controlling growth and differentiation of specific embryonic tissues.
Synonyms: Leucine-rich repeat-containing G-protein coupled receptor 5 [Precursor], Orphan G-protein coupled receptor HG38, G-protein coupled receptor 49, G-protein coupled receptor 67, LGR5, GPR49, GPR67
Molecular Weight 99867 DA
Gene ID 8549
UniProt O75473
Research Area Stem Cells, Cell Signaling, Cell Structure
Application Notes WB = 1:1000, IHC = 1:10-50, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5) (AA 443-473), (Center) antibody Western blot analysis of anti-LGR5/GPR49 Antibody (Center) (ABIN389219) in K562 cell line lysates (35 µg/lane). LGR5/GPR49(arrow) was detected using the purified polyclonal antibody (1:60dilution).
anti-Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5) (AA 443-473), (Center) antibody (2) Formalin-fixed and paraffin-embedded human brain tissue reacted with LGR5/GPR49 antibody (Center) (ABIN389219), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
anti-Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5) (AA 443-473), (Center) antibody (3) LGR5/GPR49 Antibody (Center) (ABIN389219) western blot analysis in HepG2 cell line lysates (35 µg/lane).This demonstrates the LGR5/GPR49 antibody detected the LGR5/GPR49 protein (arrow)(Kindly offered by Dr. Li).
Background publications Barker, van Es, Kuipers et al.: "Identification of stem cells in small intestine and colon by marker gene Lgr5." in: Nature, Vol. 449, Issue 7165, pp. 1003-7, 2007 (PubMed).

Validation Images
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