E2F Transcription Factor 1 (E2F1) (pSer332) antibody

Details for Product No. ABIN389869
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Antigen
Synonyms
CG6376, DRTF1/E2F, DmE2F-1, Dmel\\CG6376, Dp, E(Sev-CycE)3A, E(var)3-93E, E(var)3-95E, E(var)93E, E2F, E2F-1, E2F1, E2f1, Evar(3)164, dE2F, dE2F1, dE2f, dE2f1, de2f1, def21, drosE2F1, e2f, e2f1, l(3)0 ... show more
CG6376, DRTF1/E2F, DmE2F-1, Dmel\\CG6376, Dp, E(Sev-CycE)3A, E(var)3-93E, E(var)3-95E, E(var)93E, E2F, E2F-1, E2F1, E2f1, Evar(3)164, dE2F, dE2F1, dE2f, dE2f1, de2f1, def21, drosE2F1, e2f, e2f1, l(3)07172, l(3)j3B1, l(3)j3C2, l(3)rM729, RBAP1, RBBP3, RBP3, mKIAA4009, xE2F, ATE2FB, E2F TRANSCRIPTION FACTOR-1 E2F1, E2F transcription factor 1, E2FB, E2FB TRANSCRIPTION FACTOR show less
Epitope
pSer332
(29), (25), (21), (16), (15), (11), (7), (7), (6), (6), (6), (5), (5), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(258), (92), (58), (26), (26), (25), (12), (1), (1), (1), (1), (1)
Host
Rabbit
(208), (50), (3), (1)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(15), (12), (11), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (5)
Application
Dot Blot (DB)
(182), (87), (60), (50), (44), (39), (23), (21), (13), (5), (3), (2), (2), (1)
Pubmed 5 references available
Quantity 400 µL
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Catalog No. ABIN389869
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Immunogen This E2F1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S332 of human E2F1.
Clone RB8110
Isotype Ig
Specificity This Phospho-E2F1-S332 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S332 of human E2F1.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name E2F1
Background E2F1 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.
Synonyms: Transcription factor E2F1, E2F-1, Retinoblastoma binding protein 3, RBBP-3, PRB-binding protein E2F-1, PBR3, Retinoblastoma-associated protein 1, RBAP-1, E2F1, RBBP3
Molecular Weight 46920 DA
Gene ID 1869
UniProt Q01094
Research Area Apoptosis/Necrosis, Cancer, Cell Cycle, Phospho-specific antibodies, Transcription Factors, Signaling, Cell Structure
Application Notes DB = 1:500
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-E2F Transcription Factor 1 (E2F1) (pSer332) antibody Dot blot analysis of anti-E2F1-pS332 Pab (ABIN389869) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 µg/mL.
Background publications Saberwal, Lucas, Janssen et al.: "Increased levels and activity of E2F1 transcription factor in myelodysplastic bone marrow." in: International journal of hematology, Vol. 80, Issue 2, pp. 146-54, 2004 (PubMed).

Chaussepied, Ginsberg: "Transcriptional regulation of AKT activation by E2F." in: Molecular cell, Vol. 16, Issue 5, pp. 831-7, 2004 (PubMed).

Wang, Hou, Mohapatra et al.: "Activation of p27Kip1 Expression by E2F1. A negative feedback mechanism." in: The Journal of biological chemistry, Vol. 280, Issue 13, pp. 12339-43, 2005 (PubMed).

Joshi, Ordonez-Ercan, Dasgupta et al.: "Induction of human metallothionein 1G promoter by VEGF and heavy metals: differential involvement of E2F and metal transcription factors." in: Oncogene, Vol. 24, Issue 13, pp. 2204-17, 2005 (PubMed).

ODonnell, Wentzel, Zeller et al.: "c-Myc-regulated microRNAs modulate E2F1 expression." in: Nature, Vol. 435, Issue 7043, pp. 839-43, 2005 (PubMed).

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