Homeodomain Interacting Protein Kinase 2 (HIPK2) (pTyr361) antibody

Antigen: Homeodomain Interacting Protein Kinase 2 (HIPK2)

Synonyms: PRO0593, FLJ23711, DKFZp686K02111, HIPK2, Stank, 1110014O20Rik, B230339E18Rik, MGC152387, cb1009, MGC198433, si:dkey-16j24.2, si:dkey-239k7.1, pro0593, MGC146781

Binding Site: pTyr361

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Dot Blot (Dot), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389999

Additional Information

Alternative name: HIPK2

Gene ID: 28996

UniProt: Q9H2X6

Immunogen: This Phospho-HIPK2-pY361 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 351~380 amino acids surrounding Y361 of human HIPK2.

Isotype: Ig

Description: Other names: Homeodomain-interacting protein kinase 2,

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Phospho-HIPK2-pY361 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 351~380 amino acids surrounding Y361 of human HIPK2.

Molecular Weight: 130966 DA

Comments:

Background: HIPK2 is a conserved serine/threonine nuclear kinase that interacts with homeodomain transcription factors.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Dot Blot~~1:500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Protein Modifications

Restrictions: For Research Use only

Images

Dot blot analysis of anti-Phospho-HIPK2-pY361 Antibody (ABIN389999) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Publications

Product: Rikova, Guo, Zeng et al.: "Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer." in: Cell, Vol. 131, Issue 6, pp. 1190-203, 2007 (PubMed).

Guo, Villén, Kornhauser et al.: "Signaling networks assembled by oncogenic EGFR and c-Met." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 2, pp. 692-7, 2008 (PubMed).

Daub, Olsen, Bairlein et al.: "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." in: Molecular cell, Vol. 31, Issue 3, pp. 438-48, 2008 (PubMed).

Guha, Chaerkady, Marimuthu et al.: "Comparisons of tyrosine phosphorylated proteins in cells expressing lung cancer-specific alleles of EGFR and KRAS." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 37, pp. 14112-7, 2008 (PubMed).