Growth Factor Receptor-Bound Protein 2 (GRB2) (Center) antibody

Antigen: Growth Factor Receptor-Bound Protein 2 (GRB2)

Synonyms: ASH, Grb3-3, MST084, NCKAP2, MSTP084, EGFRBP-GRB2, Ash, AA408164, MGC108668, zgc:73077, wu:fb18e12, wu:fb34e06, GRB2, MGC127576, DKFZp459P153, grb2, MGC64880, BB233594, 8430435N19Rik, MGC112733, GRAP, MGC166297, MGC110202, zgc:110202, MGC109892, si:dkeyp-9d4.1

Epitope: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390238

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: GRB2 (Center)

Gene ID: 2885

Swiss-Prot: P62993

Immunogen: This GRB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 96~126 amino acids from the center region of human GRB2.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: Growth factor receptor-bound protein 2, Adapter protein GRB2, SH2/SH3 adapter GRB2, Protein Ash, ASH

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human GRB2.

Molecular Weight: 25206 DA

Synonyms: ASH, Grb3-3, MST084, NCKAP2, MSTP084, EGFRBP-GRB2, Ash, AA408164, MGC108668, zgc:73077, wu:fb18e12, wu:fb34e06, GRB2, MGC127576, DKFZp459P153, grb2, MGC64880, BB233594, 8430435N19Rik, MGC112733, GRAP, MGC166297, MGC110202, zgc:110202, MGC109892, si:dkeyp-9d4.1

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Stem Cells, Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of GRB2 Antibody (Center) (ABIN390238) in A2058 cell line lysates (35ug/lane). GRB2 (arrow) was detected using the purified Pab.

Western blot analysis of GRB2 (arrow) using GRB2 Antibody (Center) (ABIN390238). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GRB2 gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human brain tissue reacted with GRB2 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.