Hydroxysteroid (17-Beta) Dehydrogenase 10 (HSD17B10) (C-Term) antibody

Antigen: Hydroxysteroid (17-Beta) Dehydrogenase 10 (HSD17B10)

Synonyms: ABAD, CAMR, ERAB, HCD2, MHBD, HADH2, MRPP2, MRX17, MRX31, SCHAD, MRXS10, SDR5C1, 17b-HSD10, DUPXp11.22, HSD17, SDR9C2, EDH17B2, HSD17B10, MGC134122, hadh2, im:6899159, wu:fj61f03, zgc:101605, Hadh2

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390286

Additional Information

Alternative name: ABAD

Gene ID: 3028

UniProt: Q6IBS9

Immunogen: This ABAD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human ABAD.

Isotype: Ig

Description: Other names: hydroxyacyl-Coenzyme A dehydrogenase, type II isoform 1, 3-hydroxyacyl-CoA dehydrogenase type II, Type II HADH, 3-hydroxy-2-methylbutyryl-CoA dehydrogenase, Endoplasmic reticulum-associated amyloid beta-peptide binding protein, Short-chain type dehydrogen

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ABAD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human ABAD.

Molecular Weight: 26923 DA

Comments:

Background: Amyloid b-peptide-binding alcohol dehydrogenase (ABAD) is a member of the family of short chain dehydrogenase/reductases, unique among this family, it binds amyloid b-peptide and exhibits enzymatic activity toward a wide variety of substrates including linear alcohols. In an amyloid beta-abundant environment, ABAD appears to trigger cell stress induced by the amyloid peptide.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Metabolism, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-ABAD Pab (ABIN390286) in mouse kidney tissue lysate.ABAD(arrow) was detected using the purified Pab.

Immunohistochemistry on Formalin-fixed and paraffin-embedded human prostate tissue. (Top)Coloration with the Anti-ABAD antibody at a 1:20 concentration. (Bottom)Antibody adsorbed to the antigen (peptide), negative control.Disappearance of cytoplasmic staining indicating the antibody is specific. Data and protocol courtesy of Marie-Hélène Levesque, Centre de Recherche du CHUL, Canada.

ABAD Antibody (C214) (ABIN390286) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the ABAD antibody detected the ABAD protein (arrow).

Publications

Product: He, Merz, Mehta et al.: "Human brain short chain L-3-hydroxyacyl coenzyme A dehydrogenase is a single-domain multifunctional enzyme. Characterization of a novel 17beta-hydroxysteroid dehydrogenase." in: The Journal of biological chemistry, Vol. 274, Issue 21, pp. 15014-9, 1999 (PubMed).

Takuma, Yao, Huang et al.: "ABAD enhances Abeta-induced cell stress via mitochondrial dysfunction." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 19, Issue 6, pp. 597-8, 2005 (PubMed).