Aminolevulinate Dehydratase (ALAD) (C-Term), (AA 244-272) antibody

Details for Product No. ABIN390809
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Antigen
Synonyms DDBDRAFT_0190269, DDBDRAFT_0231415, DDB_0190269, DDB_0231415, ALAD, ncf, ALADH, PBGS, ALADR, aminolevulinatedelta-dehydratase, zgc:110219, Lv
Epitope
C-Term, AA 244-272
(11), (3), (2), (2), (1), (1), (1), (1)
Reactivity
Human
(33), (16), (14), (14), (13), (1), (1)
Host
Rabbit
(31), (3)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
(25), (9), (4), (3), (3), (3), (2)
Pubmed 1 reference available
Quantity 400 µL
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Catalog No. ABIN390809
291.50 $
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Immunogen This ALAD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 244-272 AA from the C-terminal region of human ALAD.
Clone RB20913
Isotype Ig
Specificity This ALAD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 251~280 amino acids from the C-terminal region of human ALAD.
Predicted Reactivity Monkey
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name ALAD
Background The ALAD enzyme is composed of 8 identical subunits and catalyzes the condensation of 2 molecules of delta-aminolevulinate to form porphobilinogen (a precursor of heme, cytochromes and other hemoproteins). ALAD catalyzes the second step in the porphyrin and heme biosynthetic pathway, zinc is essential for enzymatic activity. ALAD enzymatic activity is inhibited by lead and a defect in the ALAD structural gene can cause increased sensitivity to lead poisoning and acute hepatic porphyria.
Synonyms: Delta-aminolevulinic acid dehydratase, ALADH, Porphobilinogen synthase, ALAD
Molecular Weight 36295 DA
Gene ID 210
UniProt P13716
Application Notes WB = 1:1000, IHC = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Aminolevulinate Dehydratase (ALAD) (C-Term), (AA 244-272) antibody Western blot analysis of ALAD Antibody (C-term) (ABIN390809) in Jurkat cell line lysates (35 µg/lane). ALAD (arrow) was detected using the purified polyclonal antibody.
anti-Aminolevulinate Dehydratase (ALAD) (C-Term), (AA 244-272) antibody (2) Formalin-fixed and paraffin-embedded human brain tissue reacted with ALAD Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
anti-Aminolevulinate Dehydratase (ALAD) (C-Term), (AA 244-272) antibody (3) ALAD Antibody (C-term) (ABIN390809) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Guey, García-Closas, Murta-Nascimento et al.: "Genetic susceptibility to distinct bladder cancer subphenotypes." in: European urology, Vol. 57, Issue 2, pp. 283-92, 2010 (PubMed).

Validation Images
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