Aurora Kinase C (AURKC) (N-Term,Gly11) antibody

Antigen: Aurora Kinase C (AURKC)

Synonyms: AIE2, AIK3, AurC, STK13, aurora-C, AIE1, Stk13, AURKC

Binding Site: N-Term,Gly11

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN390978

Additional Information

Alternative name: Aurora-C

Gene ID: 6795

UniProt: Q9UQB9

Immunogen: This Aurora-C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human Aurora-C.

Isotype: Ig

Description: Other names: Aurora/Ipl1/Eg2 protein 2, Serine/threonine protein kinase 13, Aurora/Ipl1-related kinase 3, Aurora-C

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Aurora-C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human Aurora-C.

Molecular Weight: 35591 DA

Comments:

Background: Chromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. The Aurora kinases, members of the Ser/Thr protein kinase family, associate with microtubules during chromosome movement and segregation. Aurora kinase C may play a part in organizing microtubules in relation to the function of the centrosome/spindle pole during mitosis. This protein is localized to centrosome from anaphase to cytokinesis. Expression is limited to testis in normal cells. Elevated expression levels are seen only in a subset of cancer cells such as HepG2, HuH7 and HeLa cells. Aurora-C expression is maximum at M phase.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunofluorescence~~1:100~500 Immunoprecipitation~~Negative

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cancer, Signaling, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

The anti-Aurora C Pab (ABIN390978) is used in Western blot to detect Aurora C in lysates of 293 cells expressing Flag tag (lane 1), Flag-tagged Aurora A (lane 2), Flag-tagged Aurora B (lane 3) or Flag-tagged Aurora C (lane 4). In the immunofluorescence experiment, staining of HeLa cells expressing GFP-Aurora C is performed at different cellular mitotic stages with the anti-Aurora C Pab as primary antibody (column A), GFP fluorescence (column B), DAPI nuclear staining (column C), and anti-Aurora C merged to DAPI staining (column D). Data is kindly provided by Drs. K. Sasai and S. Sen from the University of Texas MD Anderson Cancer Center (Houston, TX).

Publications

Product: Bernard, Sanseau, Henry et al.: "Cloning of STK13, a third human protein kinase related to Drosophila aurora and budding yeast Ipl1 that maps on chromosome 19q13.3-ter." in: Genomics, Vol. 53, Issue 3, pp. 406-9, 1998 (PubMed).

Tseng, Chen, Hsu et al.: "Protein kinase profile of sperm and eggs: cloning and characterization of two novel testis-specific protein kinases (AIE1, AIE2) related to yeast and fly chromosome segregation regulators." in: DNA and cell biology, Vol. 17, Issue 10, pp. 823-33, 1998 (PubMed).

Kimura, Matsuda, Yoshioka et al.: "Cell cycle-dependent expression and centrosome localization of a third human aurora/Ipl1-related protein kinase, AIK3." in: The Journal of biological chemistry, Vol. 274, Issue 11, pp. 7334-40, 1999 (PubMed).