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LATS1 antibody (LATS, Large Tumor Suppressor, Homolog 1 (Drosophila)) (AA 1-30)

Details for Product anti-LATS1 Antibody No. ABIN391033, Supplier: Log in to see
Antigen
  • AW208599
  • Lats
  • lats1
  • RGD1564085
  • si:dkey-181i3.5
  • WARTS
  • wts
  • wu:fc19a11
  • zgc:123219
Epitope
AA 1-30, N-Term
18
16
13
13
5
3
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
71
43
42
2
Host
Rabbit
68
4
Clonality (Clone)
Polyclonal ()
Conjugate
This LATS1 antibody is un-conjugated
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
45
26
22
20
8
2
1
1
Supplier
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Immunogen This LATS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 AA from the N-terminal region of human LATS1.
Clone RB3029
Isotype Ig
Specificity This LATS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human LATS1.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name LATS1 (LATS1 Antibody Abstract)
Background The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments.
Synonyms: Serine/threonine-protein kinase LATS1, Large tumor suppressor homolog 1, WARTS protein kinase, h-warts, WARTS
Molecular Weight 126869 DA
Gene ID 9113
UniProt O95835
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cancer, Cell Cycle, Signaling, Chromatin, Cell Structure
Application Notes WB = 1:1000, IHC (p) = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-LATS1 antibody (LATS, Large Tumor Suppressor, Homolog 1 (Drosophila)) (AA 1-30) (ABIN391033) Western blot analysis of anti-LATS1 Antibody (N-term) (ABIN391033) in K562 cell line ...
Immunohistochemistry (IHC) image for anti-LATS1 antibody (LATS, Large Tumor Suppressor, Homolog 1 (Drosophila)) (AA 1-30) (ABIN391033) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
Product cited in: Eiring, Neviani, Santhanam, Oaks, Chang, Notari, Willis, Gambacorti-Passerini, Volinia, Marcucci, Caligiuri, Leone, Perrotti: "Identification of novel posttranscriptional targets of the BCR/ABL oncoprotein by ribonomics: requirement of E2F3 for BCR/ABL leukemogenesis." in: Blood, Vol. 111, Issue 2, pp. 816-28, 2008

Background publications Iida, Hirota, Morisaki, Marumoto, Hara, Kuninaka, Honda, Kosai, Kawasuji, Pallas, Saya: "Tumor suppressor WARTS ensures genomic integrity by regulating both mitotic progression and G1 tetraploidy checkpoint function." in: Oncogene, Vol. 23, Issue 31, pp. 5266-74, 2004

Yang, Yu, Hao, Li, Stewart, Insogna, Xu: "LATS1 tumour suppressor affects cytokinesis by inhibiting LIMK1." in: Nature cell biology, Vol. 6, Issue 7, pp. 609-17, 2004

Kamikubo, Takaori-Kondo, Uchiyama, Hori: "Inhibition of cell growth by conditional expression of kpm, a human homologue of Drosophila warts/lats tumor suppressor." in: The Journal of biological chemistry, Vol. 278, Issue 20, pp. 17609-14, 2003

Hisaoka, Tanaka, Hashimoto: "Molecular alterations of h-warts/LATS1 tumor suppressor in human soft tissue sarcoma." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 82, Issue 10, pp. 1427-35, 2002

Hirota, Morisaki, Nishiyama, Marumoto, Tada, Hara, Masuko, Inagaki, Hatakeyama, Saya: "Zyxin, a regulator of actin filament assembly, targets the mitotic apparatus by interacting with h-warts/LATS1 tumor suppressor." in: The Journal of cell biology, Vol. 149, Issue 5, pp. 1073-86, 2000