Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2) (Center) antibody

Antigen: Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2)

Synonyms: AMPK, AMPK2, PRKAA, A830082D05, AMPKalpha2, 2310008I11Rik, AMPKA2, prkaa1, PRKAA2, DKFZp468F2023

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391058

Additional Information

Alternative name: AMPK gamma (PRKAG1)

UniProt: AAKG_HUMAN

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human PRKAG1.

Isotype: Ig

Description: Other names: 5'-AMP-activated protein kinase, gamma-1 subunit, AMPK gamma-1 chain, AMPKg

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This PRKAG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 273~303 amino acids from the center region of human PRKAG1.

Comments:

Background: PRKAG1 is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK.

Application Details

Application Notes: The suggested dilution is: ELISA 1:1,000 Western blotting 1:100~500 Immunohistochemistry 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Metabolism, Cell Structure

Restrictions: For Research Use only

Images

The anti-PRKAG1 Pab (ABIN391058) is used in Western blot to detect PRKAG1 in mouse heart tissue lysate.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Formalin-fixed and paraffin-embedded human testis tissue reacted with PRKAG1 Antibody (Center) (ABIN391058), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Stapleton, Woollatt, Mitchelhill et al.: "AMP-activated protein kinase isoenzyme family: subunit structure and chromosomal location." in: FEBS letters, Vol. 409, Issue 3, pp. 452-6, 1997 (PubMed).

Hamilton, Stapleton, ODonnell et al.: "An activating mutation in the gamma1 subunit of the AMP-activated protein kinase." in: FEBS letters, Vol. 500, Issue 3, pp. 163-8, 2001 (PubMed).

Minokoshi, Alquier, Furukawa et al.: "AMP-kinase regulates food intake by responding to hormonal and nutrient signals in the hypothalamus." in: Nature, Vol. 428, Issue 6982, pp. 569-74, 2004 (PubMed).