|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2) (Center) antibody
|Synonyms||AMPK, AMPK2, PRKAA, A830082D05, AMPKalpha2, 2310008I11Rik, AMPKA2, prkaa1, PRKAA2, DKFZp468F2023|
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), ELISA
|3 references available|
|Quantity||0.1 mg (0.25 mg/ml) (Variants)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Alternative name||AMPK gamma (PRKAG1)|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human PRKAG1.|
|Description||Other names: 5'-AMP-activated protein kinase, gamma-1 subunit, AMPK gamma-1 chain, AMPKg|
|Characteristics||Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This PRKAG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 273~303 amino acids from the center region of human PRKAG1.|
Background: PRKAG1 is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK.
|Application Notes||The suggested dilution is: ELISA 1:1,000 Western blotting 1:100~500 Immunohistochemistry 1:50~100|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Research Area||Phospho-specific antibodies, Cell Signaling, Protein Modifications, Metabolism, Cell Structure|
|Restrictions||For Research Use only|
Stapleton, Woollatt, Mitchelhill et al.: "AMP-activated protein kinase isoenzyme family: subunit structure and chromosomal location." in: FEBS letters, Vol. 409, Issue 3, pp. 452-6, 1997 (PubMed).
Hamilton, Stapleton, ODonnell et al.: "An activating mutation in the gamma1 subunit of the AMP-activated protein kinase." in: FEBS letters, Vol. 500, Issue 3, pp. 163-8, 2001 (PubMed).
Minokoshi, Alquier, Furukawa et al.: "AMP-kinase regulates food intake by responding to hormonal and nutrient signals in the hypothalamus." in: Nature, Vol. 428, Issue 6982, pp. 569-74, 2004 (PubMed).