Doublecortin-Like Kinase 1 (DCLK1) (C-Term) antibody

Antigen: Doublecortin-Like Kinase 1 (DCLK1)

Synonyms: DCLK, DCDC3A, DCAMKL1, KIAA0369, Ania4, Cpg16, Dcamkl1, DCLK1, dcamkl1, MGC153709, wu:fc51f11, zgc:153709, MGC152477, MGC145348, Dcl, Dclk, Click-I, AI836758, mKIAA0369, 1700113D08Rik, 2810480F11Rik

Epitope: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391324

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: DCAMKL1 (C-term)

Gene ID: 9201

Swiss-Prot: O15075

Immunogen: This DCAMKL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 689~718 amino acids from the C-terminal region of human DCAMKL1.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: Serine/threonine-protein kinase DCAMKL1, Doublecortin-like and CAM kinase-like 1

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human DCAMKL1.

Molecular Weight: 82,224 DA

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:10~50 immunofluorescence

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Neurology, Cell Structure

Restrictions: For Research Use only

Images

The DCAMKL1 Antibody (C-term )(ABIN391324) western blot analysis in mouse heart tissuelysates (35ug/lane).This demonstrates the DCAMKL1 antibody detected the DCAMKL1 protein (arrow).

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with DCAMKL1 Antibody (C-term) (ABIN391324), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Expression pattern of doublecortin and CaM kinase-like-1 (DCAMKL-1) in normal mouse small intestine. (A): Immunohistochemical staining of normal small intestine for DCAMKL-1. Arrows indicate the cells positive for DCAMKL-1 in the stem cell zone. (B): Preincubation with blocking peptide completely abolished DCAMKL-1 immunoreactivity. (C): Immunohistochemical staining of normal small intestine for DCAMKL-1. Brown indicates the cells positive for DCAMKL-1 (indicated by the arrows).

Publications

Publications: Gerbe, Brulin, Makrini et al.: "DCAMKL-1 expression identifies Tuft cells rather than stem cells in the adult mouse intestinal epithelium." in: Gastroenterology, Vol. 137, Issue 6, pp. 2179-80; author reply 2180-1, 2009 (PubMed).