DCLK1 antibody (N-Term)

Catalog No. ABIN391323

Cited in 5+ publications. The Rabbit Polyclonal anti-DCLK1 antibody (Clone RB3059) (ABIN391323) specifically detects DCLK1 in WB. The antibody is reactive with Human and Mouse samples.

Quick Overview for DCLK1 antibody (N-Term) (ABIN391323)

Target: DCLK1 (Doublecortin-Like Kinase 1 (DCLK1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DCLK1 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: RB3059

Product Details anti-DCLK1 Antibody

Binding Specificity: AA 1-30, N-Term

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This DCAMKL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human DCAMKL1.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-DCLK1 antibody (ABIN391323)

Sala, Matthews, Speer, Torashima, Barthel, Grikscheit: "A multicellular approach forms a significant amount of tissue-engineered small intestine in the mouse." in: Tissue engineering. Part A, Vol. 17, Issue 13-14, pp. 1841-50, (2011) (PubMed).

Tu, Quante, Bhagat, Takaishi, Cui, Yang, Muthuplani, Shibata, Fox, Pritchard, Wang: "IFN-? inhibits gastric carcinogenesis by inducing epithelial cell autophagy and T-cell apoptosis." in: Cancer research, Vol. 71, Issue 12, pp. 4247-59, (2011) (PubMed).

Shibata, Takaishi, Muthupalani, Pritchard, Whary, Rogers, Fox, Betz, Kaestner, Karin, Wang: "Conditional deletion of IkappaB-kinase-beta accelerates helicobacter-dependent gastric apoptosis, proliferation, and preneoplasia." in: Gastroenterology, Vol. 138, Issue 3, pp. 1022-34.e1-10, (2010) (PubMed).

Jin, Ramanathan, Quante, Baik, Yang, Wang, Tu, Gordon, Pritchard, Varro, Shulkes, Wang: "Inactivating cholecystokinin-2 receptor inhibits progastrin-dependent colonic crypt fission, proliferation, and colorectal cancer in mice." in: The Journal of clinical investigation, Vol. 119, Issue 9, pp. 2691-701, (2009) (PubMed).

Gyamfi, Tanaka, He, Klaassen, Wan: "Hepatic effects of a methionine-choline-deficient diet in hepatocyte RXRalpha-null mice." in: Toxicology and applied pharmacology, Vol. 234, Issue 2, pp. 166-78, (2009) (PubMed).

Target Details for DCLK1

Target: DCLK1 (Doublecortin-Like Kinase 1 (DCLK1))

Alternative Name: DCAMKL1

Background: Doublecortin-like kinase (DCAMKL1)(Ser/Thr protein kinase family) is essential for proper neurogenesis, neuronal migration, and axonal wiring. DCAMKL1 is involved in a calcium-signaling pathway controling neuronal migration in the developing brain, and participates in functions of the mature nervous system. DCAMKL1 protein shares high homology with doublecortin (DCX). DCLK, but not DCX, is highly expressed in regions of active neurogenesis in the neocortex and cerebellum. DCAMKL1 controls mitotic division by regulating spindle formation and also determines the fate of neural progenitors during cortical neurogenesis.

Molecular Weight: 82224

Gene ID: 9201

NCBI Accession: NP_001182344, NP_001182345, NP_004725

UniProt: O15075