Mitogen-Activated Protein Kinase 10 (MAPK10) (N-Term) antibody

Antigen: Mitogen-Activated Protein Kinase 10 (MAPK10)

Synonyms: JNK3, JNK3A, PRKM10, p493F12, FLJ12099, FLJ33785, MGC50974, p54bSAPK, MAPK10, MGC123234, zgc:123234, ATMPK10, MAP kinase 10, MPK10, DKFZp459H0638

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391327

Additional Information

Alternative name: MAPK10

Gene ID: 5602

UniProt: P53779

Immunogen: This MAPK10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13~42 amino acids from the N-terminal region of human MAPK10.

Isotype: Ig

Description: Other names: Mitogen-activated protein kinase 10, Stress-activated protein kinase JNK3, MAP kinase p49 3F12

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This MAPK10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13~42 amino acids from the N-terminal region of human MAPK10.

Molecular Weight: 52585 DA

Comments:

Background: MAPK10 is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This protein is a neuronal-specific form of c-Jun N-terminal kinases (JNKs). Through its phosphorylation and nuclear localization, this kinase plays regulatory roles in the signaling pathways during neuronal apoptosis. Beta-arrestin 2, a receptor-regulated MAP kinase scaffold protein, is found to interact with, and stimulate the phosphorylation of this kinase by MAP kinase kinase 4 (MKK4). Cyclin-dependent kinase 5 can phosphorylate, and inhibit the activity of this kinase, which may be important in preventing neuronal apoptosis.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Cycle, Transcription Factors, Signaling, Neurology, Cell Structure

Restrictions: For Research Use only

Images

MAPK10 Antibody (C21) (ABIN391327) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the MAPK10 antibody detected the MAPK10 protein (arrow).

Publications

Product: Gupta, Barrett, Whitmarsh et al.: "Selective interaction of JNK protein kinase isoforms with transcription factors." in: The EMBO journal, Vol. 15, Issue 11, pp. 2760-70, 1996 (PubMed).

Yang, Kuan, Whitmarsh et al.: "Absence of excitotoxicity-induced apoptosis in the hippocampus of mice lacking the Jnk3 gene." in: Nature, Vol. 389, Issue 6653, pp. 865-70, 1997 (PubMed).

McDonald, Chow, Miller et al.: "Beta-arrestin 2: a receptor-regulated MAPK scaffold for the activation of JNK3." in: Science (New York, N.Y.), Vol. 290, Issue 5496, pp. 1574-7, 2000 (PubMed).

Yoshida, Harada, Nagai et al.: "Head-to-head juxtaposition of Fas-associated phosphatase-1 (FAP-1) and c-Jun NH2-terminal kinase 3 (JNK3) genes: genomic structure and seven polymorphisms of the FAP-1 gene." in: Journal of human genetics, Vol. 47, Issue 11, pp. 614-9, 2002 (PubMed).