|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Mitogen-Activated Protein Kinase 10 (MAPK10) (N-Term) antibody
|Synonyms||JNK3, JNK3A, PRKM10, p493F12, FLJ12099, FLJ33785, MGC50974, p54bSAPK, MAPK10, MGC123234, zgc:123234, ATMPK10, MAP kinase 10, MPK10, DKFZp459H0638|
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), ELISA
|4 references available|
|Quantity||0.1 mg (0.25 mg/ml) (Variants)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This MAPK10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13~42 amino acids from the N-terminal region of human MAPK10.|
|Description||Other names: Mitogen-activated protein kinase 10, Stress-activated protein kinase JNK3, MAP kinase p49 3F12|
|Characteristics||Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This MAPK10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13~42 amino acids from the N-terminal region of human MAPK10.|
|Molecular Weight||52585 DA|
Background: MAPK10 is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This protein is a neuronal-specific form of c-Jun N-terminal kinases (JNKs). Through its phosphorylation and nuclear localization, this kinase plays regulatory roles in the signaling pathways during neuronal apoptosis. Beta-arrestin 2, a receptor-regulated MAP kinase scaffold protein, is found to interact with, and stimulate the phosphorylation of this kinase by MAP kinase kinase 4 (MKK4). Cyclin-dependent kinase 5 can phosphorylate, and inhibit the activity of this kinase, which may be important in preventing neuronal apoptosis.
|Application Notes||The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Cycle, Transcription Factors, Signaling, Neurology, Cell Structure|
|Restrictions||For Research Use only|
|MAPK10 Antibody (C21) (ABIN391327) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the MAPK10 antibody detected the MAPK10 protein (arrow).|
Gupta, Barrett, Whitmarsh et al.: "Selective interaction of JNK protein kinase isoforms with transcription factors." in: The EMBO journal, Vol. 15, Issue 11, pp. 2760-70, 1996 (PubMed).
Yang, Kuan, Whitmarsh et al.: "Absence of excitotoxicity-induced apoptosis in the hippocampus of mice lacking the Jnk3 gene." in: Nature, Vol. 389, Issue 6653, pp. 865-70, 1997 (PubMed).
McDonald, Chow, Miller et al.: "Beta-arrestin 2: a receptor-regulated MAPK scaffold for the activation of JNK3." in: Science (New York, N.Y.), Vol. 290, Issue 5496, pp. 1574-7, 2000 (PubMed).
Yoshida, Harada, Nagai et al.: "Head-to-head juxtaposition of Fas-associated phosphatase-1 (FAP-1) and c-Jun NH2-terminal kinase 3 (JNK3) genes: genomic structure and seven polymorphisms of the FAP-1 gene." in: Journal of human genetics, Vol. 47, Issue 11, pp. 614-9, 2002 (PubMed).
|Hosts||Rabbit (42), Mouse (10)|
|Reactivities||Human (50), Mouse (Murine) (28), Rat (Rattus) (28), Chicken (19), Cow (Bovine) (19), Dog (Canine) (19), Pig (Porcine) (18), Cat (Feline) (1)|
|Applications||Western Blotting (WB) (38), ELISA (31), Immunofluorescence (IF) (22), Immunohistochemistry (IHC) (11), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (8), Immunocytochemistry (ICC) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoprecipitation (IP) (2), Flow Cytometry (FACS) (1), Immunoelectron Microscopy (IEM) (1)|
|Conjugates||Biotin (2), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||N-Term (9), C-Term (2), AA 29-44 (1), AA 380-624 (1)|