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Sphingosine Kinase 2 (SPHK2) (AA 1-30), (N-Term) antibody

Details for Product No. ABIN391348, Supplier: Log in to see
Antigen
  • CG2159
  • CG32484
  • Dmel\\CG32484
  • SK2
  • Sphk2
  • sk2
  • DKFZp469F224
  • SK 2
  • SK-2
  • SPK 2
  • SPK-2
  • C76851
Epitope
AA 1-30, N-Term
28
26
13
8
5
3
3
3
2
2
1
1
1
1
Reactivity
Human
105
38
38
Host
Rabbit
106
2
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
5
5
5
4
4
4
2
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
83
56
55
12
12
6
1
Supplier
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Immunogen This SPHK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 AA from the N-terminal region of human SPHK2.
Clone RB03623
Isotype Ig
Specificity This SPHK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 8~37 amino acids from the N-terminal region of human SPHK2.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name SPHK2 (SPHK2 Antibody Abstract)
Background Sphingosine Kinase (SphK) catalyzes the phosphorylation of the lipid sphingosine, creating the bioactive lipid sphingosine-1-phosphate (S1P). S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. SphK is an important signaling enzyme which is activated by diverse agents, including growth factors that signal through receptor tyrosine kinases, agents activating G protein-coupled receptors, and immunoglobulin receptors. Two SphK isotypes, SphK-1 and SphK-2, have been cloned, and both isotypes are ubiquitously expressed. SphK-1 has been shown to mediate cell growth, prevention of apoptosis, and cellular transformation, and is upregulated in a variety of human tumors. In contrast, SphK-2 increases apoptosis, and may be responsible for phosphorylating and activating the immunosuppressive drug FTY720.
Synonyms: Sphingosine kinase 2, SK 2, SPK 2
UniProt Q9NRA0
Research Area Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Cancer, Cell Cycle
Application Notes WB = 1:1000, IHC (p) = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Sphingosine Kinase 2 (SPHK2) (AA 1-30), (N-Term) antibody (ABIN391348) The anti-SphK2 Pab (ABIN391348) is used in Western blot (Lane 1) to detect c-myc-tagg...
Immunohistochemistry (IHC) image for anti-Sphingosine Kinase 2 (SPHK2) (AA 1-30), (N-Term) antibody (ABIN391348) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
Western Blotting (WB) image for anti-Sphingosine Kinase 2 (SPHK2) (AA 1-30), (N-Term) antibody (ABIN391348) SPHK2 Antibody (P15) (ABIN391348) western blot analysis in Ramos cell line lysates (3...
Product cited in: Sun, Zhang, Wang et al.: "Down-regulation of Sphk2 suppresses bladder cancer progression." in: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 2015 (PubMed).

Sheng, Zhang, Felice et al.: "Preconditioning stimuli induce autophagy via sphingosine kinase 2 in mouse cortical neurons." in: The Journal of biological chemistry, Vol. 289, Issue 30, pp. 20845-57, 2014 (PubMed).

Candela, Geraci, Turturici et al.: "Membrane vesicles containing matrix metalloproteinase-9 and fibroblast growth factor-2 are released into the extracellular space from mouse mesoangioblast stem cells." in: Journal of cellular physiology, Vol. 224, Issue 1, pp. 144-51, 2010 (PubMed).

Weigert, Cremer, Schmidt et al.: "Cleavage of sphingosine kinase 2 by caspase-1 provokes its release from apoptotic cells." in: Blood, Vol. 115, Issue 17, pp. 3531-40, 2010 (PubMed).

Wadgaonkar, Patel, Grinkina et al.: "Differential regulation of sphingosine kinases 1 and 2 in lung injury." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 296, Issue 4, pp. L603-13, 2009 (PubMed).

Coste, Brenneis, Linke et al.: "Sphingosine 1-phosphate modulates spinal nociceptive processing." in: The Journal of biological chemistry, Vol. 283, Issue 47, pp. 32442-51, 2008 (PubMed).

Background publications Liu, Sugiura, Nava et al.: "Molecular cloning and functional characterization of a novel mammalian sphingosine kinase type 2 isoform." in: The Journal of biological chemistry, Vol. 275, Issue 26, pp. 19513-20, 2000 (PubMed).

Shutler, Glassco, Kang et al.: "Genomic structure of the human D-site binding protein (DBP) gene." in: Genomics, Vol. 34, Issue 3, pp. 334-9, 1996 (PubMed).