Sphingosine Kinase 2 (SPHK2) (N-Term), (AA 1-30) antibody

Details for Product No. ABIN391348
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Antigen
Epitope
N-Term, AA 1-30
(13), (10), (7), (3), (3), (1), (1), (1)
Reactivity
Human
(67), (31), (29)
Host
Rabbit
(68), (1)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(52), (36), (29), (12), (10), (6), (3), (3), (2), (1)
Pubmed 2 references available
Quantity 400 μL
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Catalog No. ABIN391348
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Immunogen This SPHK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 AA from the N-terminal region of human SPHK2.
Clone RB03623
Isotype Ig
Specificity This SPHK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 8~37 amino acids from the N-terminal region of human SPHK2.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name SPHK2
Background Sphingosine Kinase (SphK) catalyzes the phosphorylation of the lipid sphingosine, creating the bioactive lipid sphingosine-1-phosphate (S1P). S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. SphK is an important signaling enzyme which is activated by diverse agents, including growth factors that signal through receptor tyrosine kinases, agents activating G protein-coupled receptors, and immunoglobulin receptors. Two SphK isotypes, SphK-1 and SphK-2, have been cloned, and both isotypes are ubiquitously expressed. SphK-1 has been shown to mediate cell growth, prevention of apoptosis, and cellular transformation, and is upregulated in a variety of human tumors. In contrast, SphK-2 increases apoptosis, and may be responsible for phosphorylating and activating the immunosuppressive drug FTY720.
Synonyms: Sphingosine kinase 2, SK 2, SPK 2
UniProt Q9NRA0
Research Area Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Cancer, Cell Cycle
Application Notes WB = 1:1000, IHC = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C.
Expiry Date 6 months
Supplier Images
anti-Sphingosine Kinase 2 (SPHK2) (N-Term), (AA 1-30) antibody The anti-SphK2 Pab (ABIN391348) is used in Western blot (Lane 1) to detect c-myc-tagged SphK2 in transfected 293 cell lysate (a c-myc antibody is used as control in Lane 2). Data is kindly provided by Dr. J. Van Brocklyn from the Ohio State University (Columbus, OH).
anti-Sphingosine Kinase 2 (SPHK2) (N-Term), (AA 1-30) antibody (2) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
anti-Sphingosine Kinase 2 (SPHK2) (N-Term), (AA 1-30) antibody (3) SPHK2 Antibody (P15) (ABIN391348) western blot analysis in Ramos cell line lysates (35 µg/lane).This demonstrates the SPHK2 antibody detected the SPHK2 protein (arrow).
Background publications Shutler, Glassco, Kang et al.: "Genomic structure of the human D-site binding protein (DBP) gene." in: Genomics, Vol. 34, Issue 3, pp. 334-9, 1996 (PubMed).

Liu, Sugiura, Nava et al.: "Molecular cloning and functional characterization of a novel mammalian sphingosine kinase type 2 isoform." in: The Journal of biological chemistry, Vol. 275, Issue 26, pp. 19513-20, 2000 (PubMed).

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