Quinone Reductase 1 (NQO1) (Center) antibody

Antigen: Quinone Reductase 1 (NQO1)

Synonyms: DTD, QR1, DHQU, DIA4, NMOR1, NMORI, Dtd, Ox1, Qr1, Dia4, Nmo1, Ox-1, Nmo-1, Nmor1, AV001255, MGC93075

Epitope: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391528

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: NQO1 (Center)

Gene ID: 1728

Swiss-Prot: P15559

Immunogen: This NQO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 124~153 amino acids from the Center region of human NQO1.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: NAD(P)H dehydrogenase [quinone] 1, Quinone reductase 1, NAD(P)H:quinone oxidoreductase 1, QR1, DT-diaphorase, DTD, Azoreductase, Phylloquinone reductase, Menadione reductase, NMOR1, DIA4, NQO1

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the Center region of human NQO1.

Molecular Weight: 30868 DA

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Flow cytometry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cancer, Metabolism, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of NQO1 antibody (Center) (ABIN391528) in NCI-H460 cell line lysates (35ug/lane). NQO1 (arrow) was detected using the purified Pab.

Phospho-OCT3/4-S136.ctrl (ABIN388790) flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.