|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Cyclin-Dependent Kinase 8 (CDK8) (N-Term) antibody
|Synonyms||K35, MGC126074, MGC126075|
Alternatives Western Blotting (WB), ELISA
|4 references available|
|Quantity||0.1 mg (0.25 mg/ml) (Variants)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This CDK8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 3~32 amino acids from the N-terminal region of human CDK8.|
|Description||Other names: Cell division protein kinase 8, Protein kinase K35|
|Characteristics||Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This CDK8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 3~32 amino acids from the N-terminal region of human CDK8.|
|Molecular Weight||53283 DA|
Background: CDK8 is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase and its regulatory subunit cyclin C are components of the RNA polymerase II holoenzyme complex, which phosphorylates the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II. This kinase has also been shown to regulate transcription by targeting the CDK7/cyclin H subunits of the general transcription initiation factor IIH (TFIIH), thus providing a link between the 'Mediator-like' protein complexes and the basal transcription machinery.
|Application Notes||The suggested dilution will be: ELISA~~1:1,000 Western blotting~~1:100~500 Flow cytometry|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Phospho-specific antibodies, Protein Modifications, Cell Cycle, Transcription Factors, Signaling, Cell Structure|
|Restrictions||For Research Use only|
|Western blot analysis of CDK8 antibody (N-term) (ABIN391759) in Jurkat cell line lysates (35ug/lane). CDK8 (arrow) was detected using the purified Pab.|
Tassan, Jaquenoud, Léopold et al.: "Identification of human cyclin-dependent kinase 8, a putative protein kinase partner for cyclin C." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 19, pp. 8871-5, 1995 (PubMed).
Rickert, Corden, Lees: "Cyclin C/CDK8 and cyclin H/CDK7/p36 are biochemically distinct CTD kinases." in: Oncogene, Vol. 18, Issue 4, pp. 1093-102, 1999 (PubMed).
Akoulitchev, Chuikov, Reinberg: "TFIIH is negatively regulated by cdk8-containing mediator complexes." in: Nature, Vol. 407, Issue 6800, pp. 102-6, 2000 (PubMed).
Di Pietro, Rapisarda, Bonaiuto et al.: "Genomics of the human genes encoding four TAFII subunits of TFIID, the three subunits of TFIIA, as well as CDK8 and SURB7." in: Somatic cell and molecular genetics, Vol. 25, Issue 3, pp. 185-9, 2001 (PubMed).
|Hosts||Rabbit (31), Mouse (1)|
|Reactivities||Human (32), Mouse (Murine) (22), Rat (Rattus) (20), Chicken (1), Dog (Canine) (1), Xenopus laevis (1), Zebrafish (1)|
|Applications||Immunofluorescence (IF) (16), Western Blotting (WB) (16), ELISA (13), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Immunohistochemistry (IHC) (2), Immunoprecipitation (IP) (2), Flow Cytometry (FACS) (1), Immunoelectron Microscopy (IEM) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||N-Term (3), C-Term (1), Center (1)|