Cyclin-Dependent Kinase 8 (CDK8) (N-Term) antibody

Antigen: Cyclin-Dependent Kinase 8 (CDK8)

Synonyms: K35, MGC126074, MGC126075

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391759

Additional Information

Alternative name: CDK8

Gene ID: 1024

UniProt: P49336

Immunogen: This CDK8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 3~32 amino acids from the N-terminal region of human CDK8.

Isotype: Ig

Description: Other names: Cell division protein kinase 8, Protein kinase K35

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This CDK8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 3~32 amino acids from the N-terminal region of human CDK8.

Molecular Weight: 53283 DA

Comments:

Background: CDK8 is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase and its regulatory subunit cyclin C are components of the RNA polymerase II holoenzyme complex, which phosphorylates the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II. This kinase has also been shown to regulate transcription by targeting the CDK7/cyclin H subunits of the general transcription initiation factor IIH (TFIIH), thus providing a link between the 'Mediator-like' protein complexes and the basal transcription machinery.

Application Details

Application Notes: The suggested dilution will be: ELISA~~1:1,000 Western blotting~~1:100~500 Flow cytometry

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Protein Modifications, Cell Cycle, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of CDK8 antibody (N-term) (ABIN391759) in Jurkat cell line lysates (35ug/lane). CDK8 (arrow) was detected using the purified Pab.

Publications

Product: Tassan, Jaquenoud, Léopold et al.: "Identification of human cyclin-dependent kinase 8, a putative protein kinase partner for cyclin C." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 19, pp. 8871-5, 1995 (PubMed).

Rickert, Corden, Lees: "Cyclin C/CDK8 and cyclin H/CDK7/p36 are biochemically distinct CTD kinases." in: Oncogene, Vol. 18, Issue 4, pp. 1093-102, 1999 (PubMed).

Akoulitchev, Chuikov, Reinberg: "TFIIH is negatively regulated by cdk8-containing mediator complexes." in: Nature, Vol. 407, Issue 6800, pp. 102-6, 2000 (PubMed).

Di Pietro, Rapisarda, Bonaiuto et al.: "Genomics of the human genes encoding four TAFII subunits of TFIID, the three subunits of TFIIA, as well as CDK8 and SURB7." in: Somatic cell and molecular genetics, Vol. 25, Issue 3, pp. 185-9, 2001 (PubMed).