CDK8 antibody (C-Term)

Catalog No. ABIN6971509

The Rabbit Polyclonal anti-CDK8 antibody (ABIN6971509) specifically detects CDK8 in WB, ChIP-seq and ChIP. The antibody is reactive with Human and Mouse samples.

Quick Overview for CDK8 antibody (C-Term) (ABIN6971509)

Target: CDK8 (Cyclin-Dependent Kinase 8 (CDK8))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK8 antibody is un-conjugated

Application: Western Blotting (WB), ChIP DNA-Sequencing (ChIP-seq), Chromatin Immunoprecipitation (ChIP)

Product Details anti-CDK8 Antibody

Binding Specificity: C-Term

Characteristics: CDK8 (Cyclin-Dependent Kinase 8) is a component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. CDK8 phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Other studies have indicated a role in transcriptional activation in response to hypoxia. CDK8 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq and Western blot, it has been shown to react with Human and Mouse samples.

Purification: Affinity Purified

Immunogen: This antibody was raised against a peptide within the C-terminal region of human CDK8.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Target Details for CDK8

Target: CDK8 (Cyclin-Dependent Kinase 8 (CDK8))

Alternative Name: CDK8

Molecular Weight: 60 kDa

NCBI Accession: NP_001251

Pathways: Cell Division Cycle, Regulation of Lipid Metabolism by PPARalpha