Acetyl-CoA Acetyltransferase 1 (ACAT1) (C-Term), (AA 296-329) antibody

Details for Product No. ABIN391807
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Antigen
Synonyms ACAT, MAT, T2, THIL, RATACAL, acat1-a, 6330585C21Rik, Acat, fd16h07, fd20g06, wu:fd16h07, wu:fd20g06, zgc:86832
Epitope
C-Term, AA 296-329
(13), (4), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(67), (36), (35), (13), (3), (2), (2), (1), (1), (1), (1), (1)
Host
Rabbit
(57), (11), (6)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(60), (36), (21), (10), (9), (5), (3), (3)
Pubmed 2 references available
Quantity 400 µL
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Catalog No. ABIN391807
291.50 $
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Immunogen This ACAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 296-329 AA from the C-terminal region of human ACAT1.
Clone RB14644
Isotype Ig
Specificity This ACAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 305~335 amino acids from the C-terminal region of human ACAT1.
Predicted Reactivity Monkey
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name ACAT1
Background ACAT1 is a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in the gene encoding ACAT1 are associated with the alpha-methylacetoaceticaciduria disorder,an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.
Synonyms: Acetyl-CoA acetyltransferase, mitochondrial [Precursor], Acetoacetyl-CoA thiolase, T2,
Molecular Weight 45200 DA
Gene ID 38
UniProt P24752
Research Area Signaling, Protein Modifications, Cell Structure
Application Notes WB = 1:1000, IHC = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Acetyl-CoA Acetyltransferase 1 (ACAT1) (C-Term), (AA 296-329) antibody Western blot analysis of anti-ACAT1 Pab (ABIN391807) in SK-BR-3 cell line lysates (35 µg/lane).ACAT1 (arrow) was detected using the purified polyclonal antibody (1:60 dilution).
anti-Acetyl-CoA Acetyltransferase 1 (ACAT1) (C-Term), (AA 296-329) antibody (2) Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ACAT1 antibody (C-term) (ABIN391807), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Background publications Haapalainen, Meriläinen, Pirilä et al.: "Crystallographic and kinetic studies of human mitochondrial acetoacetyl-CoA thiolase: the importance of potassium and chloride ions for its structure and function." in: Biochemistry, Vol. 46, Issue 14, pp. 4305-21, 2007 (PubMed).

Guo, Chang, Chang: "Functionality of the seventh and eighth transmembrane domains of acyl-coenzyme A:cholesterol acyltransferase 1." in: Biochemistry, Vol. 46, Issue 35, pp. 10063-71, 2007 (PubMed).

Validation Images
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