E2F1 antibody (AA 342-371)

Catalog No. ABIN391858

This Rabbit Polyclonal antibody specifically detects E2F1 in WB. It exhibits reactivity toward Human. It has been mentioned in 2+ publications

Quick Overview for E2F1 antibody (AA 342-371) (ABIN391858)

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E2F1 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: RB7913

Product Details anti-E2F1 Antibody

Binding Specificity: AA 342-371

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This E2F1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 342-371 amino acids from human E2F1.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:1000. WB: 1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-E2F1 antibody (ABIN391858)

Song, Gong, Yan, Hong, Wang, Wu, Li, Li, Cao: "Sj7170, a unique dual-function peptide with a specific ?-chymotrypsin inhibitory activity and a potent tumor-activating effect from scorpion venom." in: The Journal of biological chemistry, Vol. 289, Issue 17, pp. 11667-80, (2014) (PubMed).

Kim, Ju, Lim, Kang: "Maspin genetically and functionally associates with gastric cancer by regulating cell cycle progression." in: Carcinogenesis, Vol. 33, Issue 12, pp. 2344-50, (2012) (PubMed).

Target Details for E2F1

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Alternative Name: E2F1

Background: E2F1 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another two members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.

Molecular Weight: 46920

Gene ID: 1869

NCBI Accession: NP_005216

UniProt: Q01094

Pathways: p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy