EPH Receptor A7 (EPHA7) (C-Term) antibody

Antigen: EPH Receptor A7 (EPHA7)

Synonyms: EHK3, HEK11, Ebk, Ehk3, Mdk1, Cek11, Hek11, EPHA7, CEPHA7, fc51g03, wu:fc51g03, si:dkey-23k6.1

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391899

Additional Information

Alternative name: EphA7

Gene ID: 2045

UniProt: Q15375

Immunogen: This EphA7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 904~933 amino acids from the C-terminal region of human EphA7.

Isotype: Ig

Description: Other names: Ephrin type-A receptor 7, Tyrosine-protein kinase receptor EHK-3, Eph-homology kinase-3, Receptor protein-tyrosine kinase HEK11

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This EphA7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 904~933 amino acids from the C-terminal region of human EphA7.

Molecular Weight: 112096 DA

Comments:

Background: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Stem Cells, Cell Signaling, Protein Modifications, Cell Cycle, Transcription Factors, Signaling, Metabolism, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-EphA7 Pab (ABIN391899) in NCI-H460 cell lysate. EphA7 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Western blot analysis of EPHA7 (arrow) using EphA7 Antibody (C-term) (ABIN391899). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EPHA7 gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Fox, Holst, Chute et al.: "cDNA cloning and tissue distribution of five human EPH-like receptor protein-tyrosine kinases." in: Oncogene, Vol. 10, Issue 5, pp. 897-905, 1995 (PubMed).

Holder, Klein: "Eph receptors and ephrins: effectors of morphogenesis." in: Development (Cambridge, England), Vol. 126, Issue 10, pp. 2033-44, 1999 (PubMed).