|+1 404 474 4654|
|+1 888 205 9894 (TF)|
EPH Receptor B2 (EPHB2) (N-Term) antibody
|Synonyms||DRT, ERK, CAPB, Hek5, PCBC, EPHT3, Tyro5, MGC87492, RGD1564232, EPHB2|
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), ELISA
|3 references available|
|Quantity||0.1 mg (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This EphB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111~140 amino acids from the N-terminal region of human EphB2.|
|Description||Other names: Ephrin type-B receptor 2, Tyrosine-protein kinase receptor EPH-3, DRT, Receptor protein-tyrosine kinase HEK5, ERK|
|Characteristics||Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This EphB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111~140 amino acids from the N-terminal region of human EphB2.|
|Molecular Weight||117506 DA|
Background: Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The ligand-activated form of EphB2, which belongs to the Tyr family of protein kinases, interacts with multiple proteins, including GTPase-activating protein (RASGAP) through its SH2 domain. It binds RASGAP through the juxtamembrane tyrosines residues, and also interacts with PRKCABP and GRIP1 This type I membrane protein is expressed in brain, heart, lung, kidney, placenta, pancreas, liver and skeletal muscle. It is preferentially expressed in fetal brain. This protein contains putatively 2 fibronectin type III domains and 1 sterile alpha motif (SAM) domain.
|Application Notes||The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca|
|Research Area||Phospho-specific antibodies, Stem Cells, Cell Signaling, Protein Modifications, Cell Structure, Neurology|
|Restrictions||For Research Use only|
Iwase, Tanaka, Suzuki et al.: "Identification of protein-tyrosine kinase genes preferentially expressed in embryo stomach and gastric cancer." in: Biochemical and biophysical research communications, Vol. 194, Issue 2, pp. 698-705, 1993 (PubMed).
Ikegaki, Tang, Liu et al.: "Molecular characterization and chromosomal localization of DRT (EPHT3): a developmentally regulated human protein-tyrosine kinase gene of the EPH family." in: Human molecular genetics, Vol. 4, Issue 11, pp. 2033-45, 1996 (PubMed).
Thanos, Goodwill, Bowie: "Oligomeric structure of the human EphB2 receptor SAM domain." in: Science (New York, N.Y.), Vol. 283, Issue 5403, pp. 833-6, 1999 (PubMed).
|Hosts||Rabbit (30), Mouse (14), Goat (3), Rat (2)|
|Reactivities||Human (47), Mouse (Murine) (20), Chicken (18), Cow (Bovine) (18), Dog (Canine) (18), Rat (Rattus) (18)|
|Applications||ELISA (31), Western Blotting (WB) (30), Immunofluorescence (IF) (18), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (13), Flow Cytometry (FACS) (5), Immunocytochemistry (ICC) (4), Immunohistochemistry (IHC) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunohistochemistry (Paraffin-embedded Sections) pro (IHC (p)+) (2), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||C-Term (6), AA 17-200 (2), AA 27-551 (2), Internal Region (2), N-Term (2), AA 226-325 (1), AA 481-531 (1)|