EPH Receptor B2 (EPHB2) (N-Term) antibody

Antigen: EPH Receptor B2 (EPHB2)

Synonyms: DRT, ERK, CAPB, Hek5, PCBC, EPHT3, Tyro5, MGC87492, RGD1564232, EPHB2

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN391919

Additional Information

Alternative name: EphB2

Gene ID: 2048

UniProt: P29323

Immunogen: This EphB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111~140 amino acids from the N-terminal region of human EphB2.

Isotype: Ig

Description: Other names: Ephrin type-B receptor 2, Tyrosine-protein kinase receptor EPH-3, DRT, Receptor protein-tyrosine kinase HEK5, ERK

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This EphB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111~140 amino acids from the N-terminal region of human EphB2.

Molecular Weight: 117506 DA

Comments:

Background: Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The ligand-activated form of EphB2, which belongs to the Tyr family of protein kinases, interacts with multiple proteins, including GTPase-activating protein (RASGAP) through its SH2 domain. It binds RASGAP through the juxtamembrane tyrosines residues, and also interacts with PRKCABP and GRIP1 This type I membrane protein is expressed in brain, heart, lung, kidney, placenta, pancreas, liver and skeletal muscle. It is preferentially expressed in fetal brain. This protein contains putatively 2 fibronectin type III domains and 1 sterile alpha motif (SAM) domain.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Phospho-specific antibodies, Stem Cells, Cell Signaling, Protein Modifications, Cell Structure, Neurology

Restrictions: For Research Use only

Images

Western blot analysis of hEPHB2-A118 (ABIN391919) in K562 cell line lysates (35ug/lane). EPHB2 (arrow) was detected using the purified Pab.

EphB2 Antibody (N-term) (ABIN391919)immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EphB2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Publications

Product: Iwase, Tanaka, Suzuki et al.: "Identification of protein-tyrosine kinase genes preferentially expressed in embryo stomach and gastric cancer." in: Biochemical and biophysical research communications, Vol. 194, Issue 2, pp. 698-705, 1993 (PubMed).

Ikegaki, Tang, Liu et al.: "Molecular characterization and chromosomal localization of DRT (EPHT3): a developmentally regulated human protein-tyrosine kinase gene of the EPH family." in: Human molecular genetics, Vol. 4, Issue 11, pp. 2033-45, 1996 (PubMed).

Thanos, Goodwill, Bowie: "Oligomeric structure of the human EphB2 receptor SAM domain." in: Science (New York, N.Y.), Vol. 283, Issue 5403, pp. 833-6, 1999 (PubMed).