Epidermal Growth Factor Receptor (EGFR) (N-Term), (AA 27-57) antibody

Details for Product No. ABIN391929
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Antigen
Synonyms
C-erb, CG10079, D-EGFR, D-Egf, DEGFR, DER, DER flb, DER/EGFR, DER/faint little ball, DER/top, DER/torpedo, DER1, DEgfr, Degfr, Der, DmHD-33, Dmel\\CG10079, EFG-R, EGF-R, EGFR, EGFr, EGfr, EK2-6, Egf,  ... show more
C-erb, CG10079, D-EGFR, D-Egf, DEGFR, DER, DER flb, DER/EGFR, DER/faint little ball, DER/top, DER/torpedo, DER1, DEgfr, Degfr, Der, DmHD-33, Dmel\\CG10079, EFG-R, EGF-R, EGFR, EGFr, EGfr, EK2-6, Egf, Egf-r, EgfR, El, Elp, Elp-1, Elp-B1, Elp-B1RB1, HD-33, TOP, Torpedo/DER, Torpedo/Egfr, c-erbB, d-egf-r, dEGFR, dEGFR1, dEgfr, der, egfr, flb, l(2)05351, l(2)09261, l(2)57DEFa, l(2)57EFa, l(2)57Ea, mor1, top, top/DER, top/flb, torpedo/Egfr, torpedo/egfr, EGFR12, EGFR15, egfr1, Erbb2, ERBB, ERBB1, HER1, PIG61, mENA, ErbB-1, Errp, 9030024J15Rik, AI552599, Erbb, Errb1, Wa5, wa-2, wa2 show less
Epitope
N-Term, AA 27-57
(91), (58), (52), (48), (47), (44), (43), (41), (41), (39), (38), (28), (25), (23), (20), (20), (17), (17), (17), (17), (17), (15), (15), (13), (13), (13), (13), (13), (12), (12), (10), (9), (9), (8), (8), (8), (8), (8), (7), (7), (7), (6), (6), (6), (5), (5), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(1435), (673), (621), (160), (145), (124), (77), (7), (6), (3), (2), (1)
Host
Rabbit
(1129), (281), (22), (14), (8), (1), (1)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(72), (47), (36), (26), (25), (25), (25), (25), (25), (25), (25), (19), (10), (10), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(966), (431), (309), (249), (243), (173), (146), (141), (95), (70), (53), (19), (4), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 5 references available
Quantity 400 μL
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Catalog No. ABIN391929
291.50 $
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Immunogen This EGFR (ErbB1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-57 AA from the N-terminal region of human EGFR (ErbB1).
Clone RB1460
Isotype Ig
Specificity This EGFR (ErbB1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 35~65 amino acids from the N-terminal region of human ErbB1.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name EGFR (ErbB1)
Background Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.
Synonyms: Receptor protein-tyrosine kinase ErbB-1, EGFR
Molecular Weight 134277 DA
Gene ID 1956
Research Area Phospho-specific antibodies, Stem Cells, Cell Signaling, Protein Modifications, Cell Cycle, Transcription Factors, Signaling, Metabolism, Cell Structure
Application Notes WB = 1:1000, IHC = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 1.8 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Epidermal Growth Factor Receptor (EGFR) (N-Term), (AA 27-57) antibody Western blot analysis of anti-ErbB1 Pab (ABIN391929) in ZR-75-1 cell lysate. ErbB1 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
anti-Epidermal Growth Factor Receptor (EGFR) (N-Term), (AA 27-57) antibody (2) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
Background publications Huang, Jiang, Sorkin: "Tyrosine phosphorylation of the beta2 subunit of clathrin adaptor complex AP-2 reveals the role of a di-leucine motif in the epidermal growth factor receptor trafficking." in: The Journal of biological chemistry, Vol. 278, Issue 44, pp. 43411-7, 2003 (PubMed).

He, Huang, Gentry et al.: "Epidermal growth factor receptor down-regulation induced by UVA in human keratinocytes does not require the receptor kinase activity." in: The Journal of biological chemistry, Vol. 278, Issue 43, pp. 42457-65, 2003 (PubMed).

Krug, Grossmann, Schuster et al.: "Aldosterone stimulates epidermal growth factor receptor expression." in: The Journal of biological chemistry, Vol. 278, Issue 44, pp. 43060-6, 2003 (PubMed).

Hirsch, Varella-Garcia, Bunn et al.: "Epidermal growth factor receptor in non-small-cell lung carcinomas: correlation between gene copy number and protein expression and impact on prognosis." in: Journal of clinical oncology : official journal of the American Society of Clinical Oncology, Vol. 21, Issue 20, pp. 3798-807, 2003 (PubMed).

Zanardi, Yei, Lichtenstein et al.: "Distinct domains in the adenovirus E3 RIDalpha protein are required for degradation of Fas and the epidermal growth factor receptor." in: Journal of virology, Vol. 77, Issue 21, pp. 11685-96, 2003 (PubMed).

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