RYK Receptor-Like Tyrosine Kinase (RYK) antibody

Antigen: RYK Receptor-Like Tyrosine Kinase (RYK)

Synonyms: JTK5, RYK1, JTK5A, D3S3195, RYK, jtk5, ryk1, jtk5a, d3s3195, ryk, wu:fb37b12, wu:fi05b06, MGC114645

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN392050

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: RYK

Gene ID: 6259

Swiss-Prot: P34925

Immunogen: This RYK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 168~198 amino acids within aa 150~200 of human RYK.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: Tyrosine-protein kinase RYK precursor

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected within aa 150~200 of human RYK.

Molecular Weight: 67507 DA

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Protein Modifications, Cell Cycle, Signaling, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

The anti-RYK Pab (ABIN392050) is used in Western blot to detect RYK in Jurkat cell lysate.

Western blot analysis of RYK (arrow) using rabbit polyclonal RYK Antibody (ABIN392050).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RYK gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.