TEK Tyrosine Kinase, Endothelial (TEK) (C-Term) antibody

Antigen: TEK Tyrosine Kinase, Endothelial (TEK)

Synonyms: TIE2, VMCM, TIE-2, VMCM1, CD202B, MGC139569, TEK, DKFZp469E064

Epitope: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN392059

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: TEK (TIE2) (C-term)

Gene ID: 7010

Swiss-Prot: Q02763

Immunogen: This TEK (TIE2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 766~796 amino acids from the C-terminal region of human TEK.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: Angiopoietin-1 receptor, Tyrosine-protein kinase receptor TIE-2, Tyrosine-protein kinase receptor TEK, Tunica interna endothelial cell kinase, p140 TEK, CD202b, TIE2

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human TEK.

Molecular Weight: 125810 DA

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western Blot ~~ 1:50~100 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Stem Cells, Cell Signaling, Protein Modifications, Cell Cycle, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of TEK (arrow) using rabbit polyclonal TEK Antibody (C-term) (ABIN392059). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the TEK gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Formalin-fixed and paraffin-embedded human testis tissue reacted with TEK Antibody (C-term) (ABIN392059), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.