|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Interleukin-1 Receptor-Associated Kinase 1 (IRAK1) (C-Term) antibody
|Synonyms||IRAK, pelle, IRAK1, Plpk, mPLK, IRAK-1, Il1rak, IRAK1-S, AA408924, RGD1563841|
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), ELISA
|3 references available|
|Quantity||0.1 mg (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This IRAK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 683~712 amino acids from the C-terminal region of human IRAK1.|
|Description||Other names: interleukin-1 receptor-associated kinase 1|
|Characteristics||Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This IRAK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 683~712 amino acids from the C-terminal region of human IRAK1.|
|Molecular Weight||76536 DA|
Background: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine-like kinase (TLK) group consists of 40 tyrosine and serine-threonine kinases such as MLK (mixed-lineage kinase), LISK (LIMK/TESK), IRAK (interleukin-1 receptor-associated kinase), Raf, RIPK (receptor-interacting protein kinase), and STRK (activin and TGF-beta receptors) families.
|Application Notes||The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Cycle, Transcription Factors, Signaling, Metabolism, Cell Structure|
|Restrictions||For Research Use only|
Cao, Henzel, Gao: "IRAK: a kinase associated with the interleukin-1 receptor." in: Science (New York, N.Y.), Vol. 271, Issue 5252, pp. 1128-31, 1996 (PubMed).
Jiang, Johnson, Nie et al.: "Pellino 1 is required for interleukin-1 (IL-1)-mediated signaling through its interaction with the IL-1 receptor-associated kinase 4 (IRAK4)-IRAK-tumor necrosis factor receptor-associated factor 6 (TRAF6) complex." in: The Journal of biological chemistry, Vol. 278, Issue 13, pp. 10952-6, 2003 (PubMed).
Jensen, Whitehead: "Pellino3, a novel member of the Pellino protein family, promotes activation of c-Jun and Elk-1 and may act as a scaffolding protein." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 3, pp. 1500-6, 2003 (PubMed).