Interleukin-1 Receptor-Associated Kinase 1 (IRAK1) (C-Term) antibody

Antigen: Interleukin-1 Receptor-Associated Kinase 1 (IRAK1)

Synonyms: IRAK, pelle, IRAK1, Plpk, mPLK, IRAK-1, Il1rak, IRAK1-S, AA408924, RGD1563841

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN392233

Additional Information

Alternative name: IRAK

Gene ID: 3654

UniProt: P51617

Immunogen: This IRAK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 683~712 amino acids from the C-terminal region of human IRAK1.

Isotype: Ig

Description: Other names: interleukin-1 receptor-associated kinase 1

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This IRAK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 683~712 amino acids from the C-terminal region of human IRAK1.

Molecular Weight: 76536 DA

Comments:

Background: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine-like kinase (TLK) group consists of 40 tyrosine and serine-threonine kinases such as MLK (mixed-lineage kinase), LISK (LIMK/TESK), IRAK (interleukin-1 receptor-associated kinase), Raf, RIPK (receptor-interacting protein kinase), and STRK (activin and TGF-beta receptors) families.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Cycle, Transcription Factors, Signaling, Metabolism, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-IRAK1 Pab (ABIN392233) in Jurkat cell lysate. IRAK1 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Western blot analysis of IRAK1 (arrow) using rabbit polyclonal IRAK Antibody (C-term) (ABIN392233). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the IRAK1 gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Cao, Henzel, Gao: "IRAK: a kinase associated with the interleukin-1 receptor." in: Science (New York, N.Y.), Vol. 271, Issue 5252, pp. 1128-31, 1996 (PubMed).

Jiang, Johnson, Nie et al.: "Pellino 1 is required for interleukin-1 (IL-1)-mediated signaling through its interaction with the IL-1 receptor-associated kinase 4 (IRAK4)-IRAK-tumor necrosis factor receptor-associated factor 6 (TRAF6) complex." in: The Journal of biological chemistry, Vol. 278, Issue 13, pp. 10952-6, 2003 (PubMed).

Jensen, Whitehead: "Pellino3, a novel member of the Pellino protein family, promotes activation of c-Jun and Elk-1 and may act as a scaffolding protein." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 3, pp. 1500-6, 2003 (PubMed).