IRAK1 antibody (pSer376)

Catalog No. ABIN6241064

This Rabbit Polyclonal antibody specifically detects IRAK1 in WB, IF and IHC (p). It exhibits reactivity toward Human.

Quick Overview for IRAK1 antibody (pSer376) (ABIN6241064)

Target: IRAK1 (Interleukin-1 Receptor-Associated Kinase 1 (IRAK1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRAK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB56575

Product Details anti-IRAK1 Antibody

Binding Specificity: AA 348-381, pSer376

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This Phospho-IRAK1(S376) antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 348-381 amino acids from human IRAK1.

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:25. WB: 1:1000. IHC-P: 1:25. IHC-P: 1:25

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

Target Details for IRAK1

Target: IRAK1 (Interleukin-1 Receptor-Associated Kinase 1 (IRAK1))

Alternative Name: IRAK1

Background: Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor- signaling complex upon TLR activation. Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus, resulting in transcriptional activation of type I IFN genes, which drive the cell in an antiviral state. When sumoylated, translocates to the nucleus and phosphorylates STAT3.

Molecular Weight: 76537

UniProt: P51617

Pathways: NF-kappaB Signaling, TLR Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades