LIM Domain Kinase 1 (LIMK1) (N-Term), (AA 1-30) antibody

Details for Product No. ABIN392258
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Antigen
Synonyms limk1, xlimk1, GB16654, LIMK1, LIMK, LIMK-1
Epitope
N-Term, AA 1-30
(29), (9), (8), (7), (7), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(140), (73), (63), (12), (1), (1), (1)
Host
Rabbit
(103), (46)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(4), (4), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(128), (65), (34), (33), (29), (17), (10), (9), (8), (4), (3)
Pubmed 4 references available
Quantity 400 μL
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Catalog No. ABIN392258
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Immunogen This LIM Kinase 1 (LIMK1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 AA from the N-terminal region of human LIM Kinase 1 (LIMK1).
Clone RB3078
Isotype Ig
Specificity This LIMK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2~32 amino acids from the N-terminal region of human LIMK1.
Predicted Reactivity Mouse (Murine),Rat (Rattus)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name LIM Kinase 1 (LIMK1)
Background LIMK1, a member of the Ser/Thr protein kinase family, may be a component of an intracellular signaling pathway and may be involved in brain development. It phosphorylates and inactivates the actin binding/depolymerizing factor cofilin and induces actin cytoskeletal changes. The LIM domain interacts with the cytoplasmic domain of NRG1, and this cytoplasmic protein also binds ROCK1, whic phosphorylates LIMK1 on serine and/or threonine residues. Highest expression occurs in both adult and fetal nervous systems. It is detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex, and is expressed to a lesser extent in heart and skeletal muscle. Haploinsufficiency of LIMK1 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in Williams-Beuren syndrome (WBS), a rare developmental disorder. It is a contiguous gene deletion syndrome involving genes from chromosome band 7q11.23. This protein contains 2 LIM zinc-binding domains and 1 PDZ/DHR domain.
Synonyms: LIM domain kinase 1, LIMK-1
Molecular Weight 72601 DA
Gene ID 3984
UniProt P53667
Research Area Phospho-specific antibodies, Protein Modifications, Cell Structure, Transcription Factors, Signaling, Neurology
Application Notes IHC = 1:50-100, WB = 1:1000
Restrictions For Research Use only
Format Liquid
Concentration 0.45 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-LIM Domain Kinase 1 (LIMK1) (N-Term), (AA 1-30) antibody Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
anti-LIM Domain Kinase 1 (LIMK1) (N-Term), (AA 1-30) antibody (2) LIMK1 Antibody (T9) (ABIN392258) western blot analysis in SK-BR-3 cell line lysates (35 µg/lane).This demonstrates the LIMK1 antibody detected the LIMK1 protein (arrow).
Background publications Frangiskakis, Ewart, Morris et al.: "LIM-kinase1 hemizygosity implicated in impaired visuospatial constructive cognition." in: Cell, Vol. 86, Issue 1, pp. 59-69, 1996 (PubMed).

Edwards, Gill: "Structural features of LIM kinase that control effects on the actin cytoskeleton." in: The Journal of biological chemistry, Vol. 274, Issue 16, pp. 11352-61, 1999 (PubMed).

Maekawa, Ishizaki, Boku et al.: "Signaling from Rho to the actin cytoskeleton through protein kinases ROCK and LIM-kinase." in: Science (New York, N.Y.), Vol. 285, Issue 5429, pp. 895-8, 1999 (PubMed).

Ohashi, Nagata, Maekawa et al.: "Rho-associated kinase ROCK activates LIM-kinase 1 by phosphorylation at threonine 508 within the activation loop." in: The Journal of biological chemistry, Vol. 275, Issue 5, pp. 3577-82, 2000 (PubMed).

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