TBK (Ser172) antibody

Antigen: TBK

Binding Site: Ser172

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN392371

Additional Information

Gene ID: 29110

UniProt: NP_037386

Immunogen: This TBK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S172 of human TBK.

Isotype: Ig

Description: Other names: NAK, Serine/threonine-protein kinase TBK1, TANK-binding kinase 1, T2K, NF-kappa-B-activating kinase, TBK1

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This TBK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S172 of human TBK.

Molecular Weight: 83642 DA

Comments:

Background: The NF-kappa-B (NFKB) complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. TKB is similar to IKB kinases and can mediate NFKB activation in response to certain growth factors. The protein can form a complex with the IKB protein TANK and TRAF2 and release the NFKB inhibition caused by TANK.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-TBK Antibody (S172) (ABIN392371) in K562 cell line lysates (35ug/lane). TBK1(arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human testis tissue reacted with TBK-pS172, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Soulat, Bürckstümmer, Westermayer et al.: "The DEAD-box helicase DDX3X is a critical component of the TANK-binding kinase 1-dependent innate immune response." in: The EMBO journal, Vol. 27, Issue 15, pp. 2135-46, 2008 (PubMed).

Deng, Shi, Han et al.: "Negative regulation of virus-triggered IFN-beta signaling pathway by alternative splicing of TBK1." in: The Journal of biological chemistry, Vol. 283, Issue 51, pp. 35590-7, 2008 (PubMed).

Chessler, Ferreira, Chang et al.: "A novel IFN regulatory factor 3-dependent pathway activated by trypanosomes triggers IFN-beta in macrophages and fibroblasts." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 181, Issue 11, pp. 7917-24, 2008 (PubMed).