Mitogen-Activated Protein Kinase Kinase Kinase 10 (MAP3K10) (C-Term) antibody

Antigen: Mitogen-Activated Protein Kinase Kinase Kinase 10 (MAP3K10)

Synonyms: MST, MLK2, MEKK10, Mlk2, BC028668, BC046514, mlk2, xmlk2

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN392424

Additional Information

Alternative name: MLK2

Gene ID: 4294

UniProt: Q02779

Immunogen: This MLK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 636~666 amino acids from the C-terminal region of human MLK2.

Isotype: Ig

Description: Other names: Mixed lineage kinase 2, Mitogen-activated protein kinase kinase kinase 10, Protein kinase MST

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This MLK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 636~666 amino acids from the C-terminal region of human MLK2.

Molecular Weight: 103623 DA

Comments:

Background: MLK2 is a member of the serine/threonine kinase family. This kinase has been shown to activate MAPK8/JNK and MKK4/SEK1, and this kinase itself can be phoshorylated, and thus activated by JNK kinases. This kinase functions preferentially on the JNK signaling pathway, and is reported to be involved in nerve growth factor (NGF) induced neuronal apoptosis.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Cycle, Neurology, Cell Structure

Restrictions: For Research Use only

Images

The anti-MLK2 Pab (ABIN392424) is used in Western blot to detect MLK2 in transfected COS7 cell lysate. Data is kindly provided by Drs. M.K. Barthwal and A. Rana from Texas A&M University, Health Science Center (Temple, TX).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Humphrey, Newcomb, Yu et al.: "Mixed-Lineage kinase-3 stabilizes and functionally cooperates with tribbles 3 to compromise mitochondrial integrity in cytokine-induced death of pancreatic beta cells." in: The Journal of biological chemistry, 2010 (PubMed).

Product: Katoh, Hirai, Sugimura et al.: "Cloning and characterization of MST, a novel (putative) serine/threonine kinase with SH3 domain." in: Oncogene, Vol. 10, Issue 7, pp. 1447-51, 1995 (PubMed).

Dorow, Devereux, Dietzsch et al.: "Identification of a new family of human epithelial protein kinases containing two leucine/isoleucine-zipper domains." in: European journal of biochemistry / FEBS, Vol. 213, Issue 2, pp. 701-10, 1993 (PubMed).

Dorow, Devereux, Tu et al.: "Complete nucleotide sequence, expression, and chromosomal localisation of human mixed-lineage kinase 2." in: European journal of biochemistry / FEBS, Vol. 234, Issue 2, pp. 492-500, 1996 (PubMed).