|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Mitogen-Activated Protein Kinase Kinase Kinase 10 (MAP3K10) (C-Term) antibody
|Synonyms||MST, MLK2, MEKK10, Mlk2, BC028668, BC046514, mlk2, xmlk2|
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), ELISA
|4 references available|
|Quantity||0.1 mg (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This MLK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 636~666 amino acids from the C-terminal region of human MLK2.|
|Description||Other names: Mixed lineage kinase 2, Mitogen-activated protein kinase kinase kinase 10, Protein kinase MST|
|Characteristics||Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This MLK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 636~666 amino acids from the C-terminal region of human MLK2.|
|Molecular Weight||103623 DA|
Background: MLK2 is a member of the serine/threonine kinase family. This kinase has been shown to activate MAPK8/JNK and MKK4/SEK1, and this kinase itself can be phoshorylated, and thus activated by JNK kinases. This kinase functions preferentially on the JNK signaling pathway, and is reported to be involved in nerve growth factor (NGF) induced neuronal apoptosis.
|Application Notes||The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Cycle, Neurology, Cell Structure|
|Restrictions||For Research Use only|
Humphrey, Newcomb, Yu et al.: "Mixed-Lineage kinase-3 stabilizes and functionally cooperates with tribbles 3 to compromise mitochondrial integrity in cytokine-induced death of pancreatic beta cells." in: The Journal of biological chemistry, 2010 (PubMed).
Katoh, Hirai, Sugimura et al.: "Cloning and characterization of MST, a novel (putative) serine/threonine kinase with SH3 domain." in: Oncogene, Vol. 10, Issue 7, pp. 1447-51, 1995 (PubMed).
Dorow, Devereux, Dietzsch et al.: "Identification of a new family of human epithelial protein kinases containing two leucine/isoleucine-zipper domains." in: European journal of biochemistry / FEBS, Vol. 213, Issue 2, pp. 701-10, 1993 (PubMed).
Dorow, Devereux, Tu et al.: "Complete nucleotide sequence, expression, and chromosomal localisation of human mixed-lineage kinase 2." in: European journal of biochemistry / FEBS, Vol. 234, Issue 2, pp. 492-500, 1996 (PubMed).
|Reactivities||Human (29), Mouse (Murine) (21), Rat (Rattus) (18), Xenopus laevis (17), Cat (Feline) (1), Chicken (1), Cow (Bovine) (1), Dog (Canine) (1)|
|Applications||Immunofluorescence (IF) (15), Western Blotting (WB) (14), ELISA (13), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5), Immunohistochemistry (IHC) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1), Immunoprecipitation (IP) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||C-Term (4), Internal Region (1), N-Term (1), N-Term,AA 109-138 (1)|