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PI3KC3 (AA 24-53), (N-Term) antibody

Details for Product No. ABIN392555, Supplier: Log in to see
Antigen
Epitope
AA 24-53, N-Term
19
15
10
10
10
10
10
10
8
8
7
7
7
7
7
7
7
7
7
2
2
2
2
2
2
2
2
1
1
1
1
Reactivity
Human
114
1
1
1
1
1
Host
Rabbit
114
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
11
11
11
11
11
11
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
80
70
54
27
14
13
6
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Immunogen This PI3KC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-53 AA from the N-terminal region of human PI3KC3.
Clone RB1695
Isotype Ig
Specificity This PI3KC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32~61 amino acids from the N-terminal region of human autophagy kinase PI3KC3.
Predicted Reactivity Mouse (Murine),Pig (Porcine),Rat (Rattus),Xenopus laevis
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Background PI3KC3 is a catalytic subunit of the PI3K complex involved in the transport of lysosomal enzyme precursors to lysosomes. This enzyme acts catalytically to convert 1-phosphatidyl-1D-myo-inositol to 1-phosphatidyl-1D-myo-inositol 3-phosphate. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). The regulation of the Beclin 1-PI3KC3 complex lipid kinase activity is a critical element in the autophagy signaling pathway.
Synonyms: Phosphatidylinositol 3-kinase catalytic subunit type 3, PtdIns-3-kinase type 3, PI3-kinase type 3, PI3K type 3, Phosphoinositide-3-kinase class 3, Phosphatidylinositol 3-kinase p100 subunit, vps34, PI 3 Kinase Class 3
Molecular Weight 101549 DA
Gene ID 5289
UniProt Q8NEB9
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Autophagy
Application Notes WB = 1:1000, IHC (p) = 1:50-100, IF = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 1.64 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-PI3KC3 (AA 24-53), (N-Term) antibody (ABIN392555) Western blot analysis of anti-PI3KC3 Pab (ABIN392555) in HeLa cell lysate. PI3KC3 (ar...
Immunohistochemistry (IHC) image for anti-PI3KC3 (AA 24-53), (N-Term) antibody (ABIN392555) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
Product cited in: Zhang, Morgan, Chen, Choksi, Liu: "Induction of autophagy is essential for monocyte-macrophage differentiation." in: Blood, Vol. 119, Issue 12, pp. 2895-905, 2012

McLeod, Zhou, Li, Wang, He: "The class III kinase Vps34 promotes T lymphocyte survival through regulating IL-7R? surface expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 187, Issue 10, pp. 5051-61, 2011

Zhou, Wang, Hasegawa, Amin, Han, Kaneko, He, Wang: "Deletion of PIK3C3/Vps34 in sensory neurons causes rapid neurodegeneration by disrupting the endosomal but not the autophagic pathway." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 20, pp. 9424-9, 2010

Wang, Whiteman, Lian, Wang, Singh, Huang, Denmark: "A non-canonical MEK/ERK signaling pathway regulates autophagy via regulating Beclin 1." in: The Journal of biological chemistry, Vol. 284, Issue 32, pp. 21412-24, 2009

Miselli, Negri, Gronchi, Losa, Conca, Brich, Fumagalli, Fiore, Casali, Pierotti, Tamborini, Pilotti: "Is autophagy rather than apoptosis the regression driver in imatinib-treated gastrointestinal stromal tumors?" in: Translational oncology, Vol. 1, Issue 4, pp. 177-86, 2008

Liang, Feng, Ku, Dotan, Canaani, Oh, Jung: "Autophagic and tumour suppressor activity of a novel Beclin1-binding protein UVRAG." in: Nature cell biology, Vol. 8, Issue 7, pp. 688-99, 2006

Background publications Vergne, Chua, Deretic: "Tuberculosis toxin blocking phagosome maturation inhibits a novel Ca2+/calmodulin-PI3K hVPS34 cascade." in: The Journal of experimental medicine, Vol. 198, Issue 4, pp. 653-9, 2003

Volinia, Dhand, Vanhaesebroeck, MacDougall, Stein, Zvelebil, Domin, Panaretou, Waterfield: "A human phosphatidylinositol 3-kinase complex related to the yeast Vps34p-Vps15p protein sorting system." in: The EMBO journal, Vol. 14, Issue 14, pp. 3339-48, 1995