Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Predicted Reactivity
M, Pig, Rat
Purification
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen
This PIP4K2 Alpha (PIP4K2A) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 303-335 amino acids from the C-terminal region of human PIP4K2 Alpha (PIP4K2A).
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date
6 months
Jones, Foulger, Keune, Bultsma, Divecha: "PtdIns5P is an oxidative stress-induced second messenger that regulates PKB activation." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 27, Issue 4, pp. 1644-56, (2013) (PubMed).
Keune, Jones, Bultsma, Sommer, Zhou, Lu, Divecha: "Regulation of phosphatidylinositol-5-phosphate signaling by Pin1 determines sensitivity to oxidative stress." in: Science signaling, Vol. 5, Issue 252, pp. ra86, (2012) (PubMed).
Huang, Anderson, Cao, Wiechmann, Rajala: "Light-induced tyrosine phosphorylation of rod outer segment membrane proteins regulate the translocation, membrane binding and activation of type II ? phosphatidylinositol-5-phosphate 4-kinase." in: Neurochemical research, Vol. 36, Issue 4, pp. 627-35, (2011) (PubMed).
Bultsma, Keune, Divecha: "PIP4Kbeta interacts with and modulates nuclear localization of the high-activity PtdIns5P-4-kinase isoform PIP4Kalpha." in: The Biochemical journal, Vol. 430, Issue 2, pp. 223-35, (2010) (PubMed).
Wilcox, Hinchliffe: "Regulation of extranuclear PtdIns5P production by phosphatidylinositol phosphate 4-kinase 2alpha." in: FEBS letters, Vol. 582, Issue 9, pp. 1391-4, (2008) (PubMed).
Target
PIP4K2A
(Phosphatidylinositol-5-Phosphate 4-Kinase, Type II, alpha (PIP4K2A))
PI5P4KA antibody, PIP5K2A antibody, PIP5KII-alpha antibody, PIP5KIIA antibody, PIPK antibody, AW742916 antibody, Pip5k2a antibody, pipk antibody, pi5p4ka antibody, pip5k2a antibody, pip5kiia antibody, pip4k2a antibody, zgc:194746 antibody, zgc:194777 antibody, phosphatidylinositol-5-phosphate 4-kinase type 2 alpha antibody, phosphatidylinositol-5-phosphate 4-kinase, type II, alpha antibody, phosphatidylinositol-5-phosphate 4-kinase, type II, alpha S homeolog antibody, phosphatidylinositol-5-phosphate 4-kinase, type II, alpha a antibody, PIP4K2A antibody, Pip4k2a antibody, pip4k2a.S antibody, pip4k2a antibody, pip4k2aa antibody
Background
Phosphatidylinositol-4,5-bisphosphate, the precursor to second messengers of the phosphoinositide signal transduction pathways, is thought to be involved in the regulation of secretion, cell proliferation, differentiation, and motility. TPIP5K2A is one of a family of enzymes capable of catalyzing the phosphorylation of phosphatidylinositol-4-phosphate on the fifth hydroxyl of the myo-inositol ring to form phosphatidylinositol-4,5-bisphosphate. The amino acid sequence of this enzyme does not show homology to other kinases, but the recombinant protein does exhibit kinase activity. The protein is a member of the phosphatidylinositol-4-phosphate 5-kinase family.