NIMA (Never in Mitosis Gene A)- Related Kinase 9 (NEK9) (C-Term) antibody

Antigen: NIMA (Never in Mitosis Gene A)- Related Kinase 9 (NEK9)

Synonyms: Nek8, NERCC, NERCC1, MGC16714, MGC138306, DKFZp434D0935, jck, nphp9, nek12a, MGC88903, NEK9, MGC80499, nercc, nercc1

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN392654

Additional Information

Alternative name: NEK9

Gene ID: 91754

UniProt: Q8TD19

Immunogen: This NEK9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 855~884 amino acids from the C-terminal region of human NEK9.

Isotype: Ig

Description: Other names: Serine/threonine-protein kinase Nek9, NimA-related protein kinase 9, Nercc1 kinase, NIMA-related kinase 8

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This NEK9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 855~884 amino acids from the C-terminal region of human NEK9.

Molecular Weight: 107149 DA

Comments:

Background: NEK9, a member of the NIMA subfamily of Ser/Thr protein kinases, is a pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. It phosphorylates different histones (serine and threonine residues on H3), myelin basic protein, beta-casein (serine residues), and BICD2. NEK9 is activated during mitosis by intramolecular autophosphorylation. Activity and autophosphorylation is activated by manganese >> magnesium ions, and is sensitive to increasing concentration of detergents. This protein is not cell-cycle regulated but activity is higher in G0-arrested cells. NEK is part of a homodimer that binds to Ran GTPase, and exhibits a greater affinity for Ran-GDP over Ran-GTP. Interaction is also noted with NEK6 and NEK7 family members. This cytoplasmic protein is most abundant in heart, liver, kidney and testis, and is also expressed in smooth muscle cells and fibroblasts. Expression varies mildly across the cell cycle, with highest expression observed in G1 and stationary-phase cells.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Protein Modifications, Cell Cycle, Signaling, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

The anti-NEK9 Pab (ABIN392654) is used in Western blot to detect NEK9 in 293 cell lysate (Lane 1) and mouse heart tissue lysate (Lane 2).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Holland, Milne, Garka et al.: "Purification, cloning, and characterization of Nek8, a novel NIMA-related kinase, and its candidate substrate Bicd2." in: The Journal of biological chemistry, Vol. 277, Issue 18, pp. 16229-40, 2002 (PubMed).

Roig, Mikhailov, Belham et al.: "Nercc1, a mammalian NIMA-family kinase, binds the Ran GTPase and regulates mitotic progression." in: Genes & development, Vol. 16, Issue 13, pp. 1640-58, 2002 (PubMed).

Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Belham, Roig, Caldwell et al.: "A mitotic cascade of NIMA family kinases. Nercc1/Nek9 activates the Nek6 and Nek7 kinases." in: The Journal of biological chemistry, Vol. 278, Issue 37, pp. 34897-909, 2003 (PubMed).

Tan, Lee: "Nek9, a novel FACT-associated protein, modulates interphase progression." in: The Journal of biological chemistry, Vol. 279, Issue 10, pp. 9321-30, 2004 (PubMed).