PTPN5 antibody (N-Term)
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- Target See all PTPN5 Antibodies
- PTPN5 (Protein tyrosine Phosphatase, Non-Receptor Type 5 (Striatum-Enriched) (PTPN5))
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Binding Specificity
- AA 168-198, N-Term
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Reactivity
- Human, Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This PTPN5 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purification
- This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
- Immunogen
- This STEP / PTPN5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 168-198 amino acids from the N-terminal region of human STEP / PTPN5.
- Clone
- RB00604
- Isotype
- Ig Fraction
- Top Product
- Discover our top product PTPN5 Primary Antibody
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- Application Notes
- WB: 1:1000. WB: 1:1000. IHC-P: 1:10~50
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
- Expiry Date
- 6 months
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- Target
- PTPN5 (Protein tyrosine Phosphatase, Non-Receptor Type 5 (Striatum-Enriched) (PTPN5))
- Alternative Name
- STEP / PTPN5 (PTPN5 Products)
- Synonyms
- STEP antibody, Step antibody, PTPSTEP antibody, protein tyrosine phosphatase, non-receptor type 5 antibody, ptpn5 antibody, PTPN5 antibody, Ptpn5 antibody
- Background
- STEP (striatum-enriched phosphatase) is a neural-specific protein-tyrosine phosphatase, first isolated from the rat brain. The 537-amino acid predicted human protein as isolated from cDNA sequences is between 85 and 90 % identical to the mouse and rat sequences. In rat neuronal cell cultures, glutamate-mediated activation of N-methyl-D-aspartate (NMDA) receptors leads to the rapid but transient phosphorylation of extracellular signal-related kinase-2 (ERK2). NMDA-mediated influx of calcium, activates the calcium-dependent phosphatase calcineurin and the resulting dephosphorylation and activation of STEP. STEP then inactivatea ERK2 through tyrosine dephosphorylation and blocks translocation of the kinase to the nucleus. STEP plays a significant role in regulating the ERK activation and downstream signaling in neurons.
- Molecular Weight
- 63538
- Gene ID
- 84867
- NCBI Accession
- NP_001035059, NP_001265165, NP_001265167, NP_001265168, NP_008837, NP_116170
- UniProt
- P54829
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