ELISA: 1/5,000 - 1/20,000. Western blot: 1/500 - 1/2,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.7 mg/mL (by UV absorbance at 280 nm)
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
-20 °C
Target
AP2A2
(Adaptor-Related Protein Complex 2, alpha 2 Subunit (AP2A2))
MGC99219 antibody, ADTAB antibody, CLAPA2 antibody, HIP-9 antibody, HIP9 antibody, HYPJ antibody, 2410074K14Rik antibody, AF006990 antibody, AW146353 antibody, Adtab antibody, C78001 antibody, L25 antibody, adaptor related protein complex 2 alpha 2 subunit antibody, adaptor related protein complex 2 alpha 2 subunit L homeolog antibody, adaptor-related protein complex 2, alpha 2 subunit antibody, AP2A2 antibody, ap2a2.L antibody, Ap2a2 antibody
Background
The AP2A1 and AP2A2 represent the AP2 alpha proteins that are found in the AP2 complex in clathrincoated vesicles. AP2A is also known as Alpha-adaptin A, Adaptor protein complex AP-2 alpha-1 subunit, Clathrin assembly protein complex 2 alpha-A large chain, 100 kDa coated vesicle protein A and Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit. The AP2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles, which link clathrin to receptors in vesicles.Synonyms: 100 kDa coated vesicle protein C, ADTAB, AP-2 complex subunit alpha-2, Adapter-related protein complex 2 alpha-2 subunit, Adaptor protein complex AP-2 subunit alpha-2, Alpha-2-adaptin, Alpha-adaptin C, CLAPA2, HYPJ, Huntingtin yeast partner J, Huntingtin-interacting protein 9, Huntingtin-interacting protein J, KIAA0899, Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit, lathrin assembly protein complex 2 alpha-C large chain